Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Apr 16;128(1):331-8.
doi: 10.1016/0006-291x(85)91683-3.

Binding to erythrocyte membrane is the physiological mechanism for activation of Ca2+-dependent neutral proteinase

S Pontremoli, E Melloni, B Sparatore, F Salamino, M Michetti, O Sacco, B L Horecker

Binding to erythrocyte membrane is the physiological mechanism for activation of Ca2+-dependent neutral proteinase

S Pontremoli et al. Biochem Biophys Res Commun. .

Abstract

In the presence of micromolar concentrations of Ca2+ the catalytic 80 kDa subunit of human erythrocyte procalpain binds to the cytosolic surface of the erythrocyte membrane. Binding is rapid, highly specific and is reversed by the removal of Ca2+. In the bound form the 80 kDa catalytic subunit undergoes a rapid conversion to calpain, the active 75 kDa Ca2+-requiring proteinase. The activated proteinase produces extensive degradation of membrane components, particularly of band 4.1 and 2.1 proteins. Binding to membranes may represent an obligatory physiological mechanism for the conversion of procalpain to calpain.

PubMed Disclaimer

Publication types

LinkOut - more resources