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Review
. 2018 Apr;122(1):e59.
doi: 10.1002/cpmb.59.

Overview of Next-Generation Sequencing Technologies

Barton E Slatko  1 , Andrew F Gardner  1 , Frederick M Ausubel  2
Affiliations
Review

Overview of Next-Generation Sequencing Technologies

Barton E Slatko et al. Curr Protoc Mol Biol. 2018 Apr.

Abstract

High throughput DNA sequencing methodology (next generation sequencing; NGS) has rapidly evolved over the past 15 years and new methods are continually being commercialized. As the technology develops, so do increases in the number of corresponding applications for basic and applied science. The purpose of this review is to provide a compendium of NGS methodologies and associated applications. Each brief discussion is followed by web links to the manufacturer and/or web-based visualizations. Keyword searches, such as with Google, may also provide helpful internet links and information. © 2018 by John Wiley & Sons, Inc.

Keywords: NGS; Sanger sequencing; next-generation sequencing.

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References

    1. Ammar R, Paton TA, Torti D, Shlien A, Bader GD. Long read nanopore sequencing for detection of HLA and CYP2D6 variants and haplotypes. F1000Res. 2015;4:17. doi: 10.12688/f1000research.6037.2. - DOI - PMC - PubMed
    1. Benner S, Chen RJ, Wilson NA, Abu-Shumays R, Hurt N, Lieberman KR, … Akeson M. Sequence-specific detection of individual DNA polymerase complexes in real time using a nanopore. Nat Nanotechnol. 2007;2(11):718–724. doi: 10.1038/nnano.2007.344. - DOI - PMC - PubMed
    1. Bogas D, Nyberg L, Pacheco R, Azevedo NF, Beech JP, Gomila M, … Westerlund F. Applications of optical DNA mapping in microbiology. Biotechniques. 2017;62(6):255–267. doi: 10.2144/000114555. - DOI - PubMed
    1. Branton D, Deamer DW, Marziali A, Bayley H, Benner SA, Butler T, … Schloss JA. The potential and challenges of nanopore sequencing. Nat Biotechnol. 2008;26(10):1146–1153. doi: 10.1038/nbt.1495. - DOI - PMC - PubMed
    1. Cai W, Aburatani H, Stanton VP, Jr, Housman DE, Wang YK, Schwartz DC. Ordered restriction endonuclease maps of yeast artificial chromosomes created by optical mapping on surfaces. Proc Natl Acad Sci U S A. 1995;92(11):5164–5168. - PMC - PubMed

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