Multiplex PCR reveals high prevalence of enterovirus and HHV6 in acellular paediatric cerebrospinal fluid samples

Sheila F Lumley¹, Dave Pritchard², Atanu Dutta³, Philippa C Matthews⁴, Kathy Cann²

Affiliations

¹ Microbiology Department, Stoke Mandeville Hospital, Buckinghamshire Healthcare NHS Trust, Mandeville Road, Bucks HP21 8AL, UK; Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Road, Oxford OX1 3SY, UK; Microbiology and Infectious Disease, Oxford University Hospitals NHS Foundation Trust, Headington, Oxford OX3 9DU, UK. Electronic address: Sheila.lumley@nhs.net.
² Microbiology Department, Stoke Mandeville Hospital, Buckinghamshire Healthcare NHS Trust, Mandeville Road, Bucks HP21 8AL, UK.
³ Paediatrics Department, Stoke Mandeville Hospital, Buckinghamshire Healthcare NHS Trust, Mandeville Road, Bucks HP21 8AL, UK.
⁴ Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Road, Oxford OX1 3SY, UK; Microbiology and Infectious Disease, Oxford University Hospitals NHS Foundation Trust, Headington, Oxford OX3 9DU, UK.

PMID: 29852190
DOI: 10.1016/j.jinf.2018.05.008

Comment

Multiplex PCR reveals high prevalence of enterovirus and HHV6 in acellular paediatric cerebrospinal fluid samples

Sheila F Lumley et al. J Infect. 2018 Sep.

. 2018 Sep;77(3):249-257.

doi: 10.1016/j.jinf.2018.05.008. Epub 2018 May 29.

Authors

Sheila F Lumley¹, Dave Pritchard², Atanu Dutta³, Philippa C Matthews⁴, Kathy Cann²

Affiliations

¹ Microbiology Department, Stoke Mandeville Hospital, Buckinghamshire Healthcare NHS Trust, Mandeville Road, Bucks HP21 8AL, UK; Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Road, Oxford OX1 3SY, UK; Microbiology and Infectious Disease, Oxford University Hospitals NHS Foundation Trust, Headington, Oxford OX3 9DU, UK. Electronic address: Sheila.lumley@nhs.net.
² Microbiology Department, Stoke Mandeville Hospital, Buckinghamshire Healthcare NHS Trust, Mandeville Road, Bucks HP21 8AL, UK.
³ Paediatrics Department, Stoke Mandeville Hospital, Buckinghamshire Healthcare NHS Trust, Mandeville Road, Bucks HP21 8AL, UK.
⁴ Nuffield Department of Medicine, Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Road, Oxford OX1 3SY, UK; Microbiology and Infectious Disease, Oxford University Hospitals NHS Foundation Trust, Headington, Oxford OX3 9DU, UK.

PMID: 29852190
DOI: 10.1016/j.jinf.2018.05.008

No abstract available

Keywords: Biofire FilmArray; Cell count; Cerebrospinal fluid; Encephalitis; Meningitis; PCR; Pleocytosis.

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A case of a FilmArray^® ME false negative in meningococcal meningitis.
González-Donapetry P, García-Rodríguez J, Cendejas-Bueno E. González-Donapetry P, et al. J Infect. 2019 Sep;79(3):277-287. doi: 10.1016/j.jinf.2019.05.002. Epub 2019 May 15. J Infect. 2019. PMID: 31102602
Paediatric enterovirus meningitis without cerebrospinal fluid pleocytosis.
Alhazmi A, Lazrek M, Alidjinou EK, Descombes G, Engelmann I, Hober D. Alhazmi A, et al. J Infect. 2019 Dec;79(6):612-625. doi: 10.1016/j.jinf.2019.11.006. Epub 2019 Nov 13. J Infect. 2019. PMID: 31733234 No abstract available.

Comment on

Diagnostic usefulness of Xpert MTB/RIF assay for detection of tuberculous meningitis using cerebrospinal fluid.
Rufai SB, Singh A, Singh J, Kumar P, Sankar MM, Singh S; TB Research Team. Rufai SB, et al. J Infect. 2017 Aug;75(2):125-131. doi: 10.1016/j.jinf.2017.04.010. Epub 2017 May 10. J Infect. 2017. PMID: 28501491

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Multiplex PCR reveals high prevalence of enterovirus and HHV6 in acellular paediatric cerebrospinal fluid samples

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Multiplex PCR reveals high prevalence of enterovirus and HHV6 in acellular paediatric cerebrospinal fluid samples

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