High-frequency transduction of c-erbB in avian leukosis virus-induced erythroblastosis

Abstract

Twenty-one cases of Rous-associated virus type 1-induced erythroblastosis have been analyzed for novel restriction endonuclease fragments of c-erbB. Twenty of the erythroleukemias contained novel c-erbB fragments; 10 of these were found to contain a proviral insertion in c-erbB, and 10 were found to have a new transduction of c-erbB. Each of the proviral insertions was in the same transcriptional orientation as c-erbB, and most appeared to have retained both long terminal repeats as well as 5' viral sequences that signal packaging of RNA into virions. Each of the new c-erbB transducing viruses had a characteristic EcoRI fragment that contained a spliced form of c-erbB sequences. When inoculated into 1-week-old chickens, the new transducing viruses caused rapid-onset erythroblastosis.