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. 1985 May;82(9):2844-6.
doi: 10.1073/pnas.82.9.2844.

A drug-resistant mutation in the ribosomal DNA of Tetrahymena

P J Bruns, A L Katzen, L Martin, E H Blackburn

A drug-resistant mutation in the ribosomal DNA of Tetrahymena

P J Bruns et al. Proc Natl Acad Sci U S A. 1985 May.

Abstract

A mutation that confers resistance to the drug paromomycin is shown to be in the structural gene that codes for the ribosomal RNA in Tetrahymena. This observation was made by exploiting a variant of the ribosomal DNA that distorts amplification of this locus when a new somatic nucleus develops during conjugation. Because the allelic forms of this locus have a restriction endonuclease site polymorphism, it was possible to correlate drug resistance with presence of a specific allele. The genetic results have been confirmed by sequence analysis (presented elsewhere). Thus, the crosses presented here provide a unique opportunity to identify mutations in the ribosomal DNA.

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