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. 2018 May 15:2018:2186027.
doi: 10.1155/2018/2186027. eCollection 2018.

Genetic Characterization of Animal Brucella Isolates from Northwest Region in China

Xiaoan Cao  1 , Youjun Shang  1 , Yongsheng Liu  1 , Zhaocai Li  1 , Zhizhong Jing  1
Affiliations

Genetic Characterization of Animal Brucella Isolates from Northwest Region in China

Xiaoan Cao et al. Biomed Res Int. .

Abstract

Animal brucellosis is a reemerging disease in China, particular in northwest China. The Brucella species (even genus) are highly conserved; therefore the use of Multilocus sequencing typing (MLST: based on conserved housekeeping loci) is more suitable for discrimination at species or biovar level on Brucella. In this study, MLST was used to analyze the characterization of Brucella from sheep and yaks during 2015 and 2016. All 66 isolates were collected from northwest China, including Inner Mongolia, Xinjiang, Qinghai, and Gansu provinces. Isolates were cultured on Brucella agar medium and identified by MLST. MLST identified five ST types: ST8 (n = 55), ST7 (n = 2), ST3 (n = 5), ST1 (n = 2), and ST14 (n = 2). This analysis revealed that B. melitensis isolates exhibited high single genotypes (ST8) in the most northwest China. MLST of isolates provides helpful information on understanding genetic characterization of Brucella in northwest China.

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Figures

Figure 1
Figure 1
Dendrogram based on the MLST genotyping assay showing relationships of 66 Brucella isolates. Key: serial number for the 66 isolates; ST: MLST genotype fo isolates; strain: the number conferred to isolates; host: the hosts from which the bacteria were isolated; location: sample specific location (Table 1 in detail); province: the regions of sample collection; year: the time of isolation of bacteria.
Figure 2
Figure 2
Geographic distribution of Brucella genotype ST8 in northwest China. Dotted (red) countries: sampling area (Table 1 in detail); other shapes: the other data collected in different provinces in northwest China from [–14].
See this image and copyright information in PMC

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