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. 1985 Feb 1;144(2):577-83.
doi: 10.1016/0003-2697(85)90156-3.

The basolateral membrane of rat enterocyte: its purification from brush border contamination

M N Orsenigo, M Tosco, G Esposito, A Faelli

The basolateral membrane of rat enterocyte: its purification from brush border contamination

M N Orsenigo et al. Anal Biochem. .

Abstract

Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaCl2 to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low L-glucose permeability across basolateral membrane vesicles together with ATP-dependent sodium uptake was observed.

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