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. 2018:1798:25-37.
doi: 10.1007/978-1-4939-7893-9_3.

Encapsulation of Exogenous Proteins in Vault Nanoparticles

Meng Wang  1 , Danny Abad  2 , Valerie A Kickhoefer  2 , Leonard H Rome  2 , Shaily Mahendra  3
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Encapsulation of Exogenous Proteins in Vault Nanoparticles

Meng Wang et al. Methods Mol Biol. 2018.

Abstract

Natural vault nanoparticles are ribonucleoprotein particles with a mass of 13 MDa that have been found in a wide variety of eukaryotes. Empty recombinant vaults are assembled from heterologously expressed Major Vault Protein (MVP), forming the barrel-shaped vault shell. These structures are morphologically indistinguishable from natural vault particles. Here, we describe the packaging and purification of exogenous proteins into these recombinant vault particles by mixing with proteins attached to the INT domain that binds to MVP.

Keywords: Baculovirus expression; Enzyme immobilization; Nanocages; Nanostructures; Protein scaffold.

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