Effect of PEDOT:PSS in tissue engineering composite scaffold on improvement and maintenance of endothelial cell function

Abstract

According to recent research, smart polymers can affect different kind of mammalian cells such as endothelial cells. It is known that conductive polymers have great features, e.g. electrical conductivity, and can help increase electrical cell communication. To clarify the effect of one of these smart materials on endothelial cells, which are not inherently electrically dependent, poly(3, 4-ethylene dioxythiophene) polystyrene sulfonate (PEDOT:PSS) was chosen. Scaffolds were composed of gelatin, alginate, and PEDOT:PSS and made through solvent casting. Human umbilical vein endothelial cells (HUVECs) were cultured on the scaffold with different PEDOT:PSS concentrations. SEM, MTT assay, cell attachment, nitric oxide measurement, real-time PCR and immunohistochemistry analysis were employed to assess endothelial cell responses. Although there was no significant difference in swelling ratio, mass loss, and cell attachment when PEDOT:PSS concentration increased in scaffold construction, cell proliferation noticeably increased after seven days. The cells showed a significant increase in proliferation and NO release to the scaffold with 1% PEDOT:PSS concentration. The results indicated increases in the amount of expression of platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31), kinase insert domain receptor (KDR), vascular-endothelial Cadherin (VE. Cadherin), and von Will brand factor (vWf) in the group which contained a conductive polymer in comparison with the non-conductive scaffold. Therefore, as a conductive polymer, PEDOT:PSS can affect the endothelial cell behaviours.