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. 1985 Jun;105(6):700-5.

Enzyme immunoassay of anti-glomerular basement membrane antibodies

A J Fish, M Kleppel, K Jeraj, A F Michael
  • PMID: 2987380

Enzyme immunoassay of anti-glomerular basement membrane antibodies

A J Fish et al. J Lab Clin Med. 1985 Jun.

Abstract

An immunoassay has been developed to detect anti-glomerular basement membrane (GBM) antibodies in human sera. Various plating conditions, types of microtiter plates, and the use of biotinylated or peroxidase-labeled secondary antibodies were examined. The described assay is reliable, fast, and convenient. Sera with positive reactivity in anti-GBM nephritis and Goodpasture's syndrome are readily discriminated from sera obtained from normal individuals or patients with a variety of other diseases.

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