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. 2018 Jun 6;13(6):e0197044.
doi: 10.1371/journal.pone.0197044. eCollection 2018.

Anticancer, antioxidant, and antibacterial activities of low molecular weight bioactive subfractions isolated from cultures of wood degrading fungus Cerrena unicolor

Anna Matuszewska  1 , Magdalena Jaszek  1 , Dawid Stefaniuk  1 , Tomasz Ciszewski  2 , Łukasz Matuszewski  3
Affiliations

Anticancer, antioxidant, and antibacterial activities of low molecular weight bioactive subfractions isolated from cultures of wood degrading fungus Cerrena unicolor

Anna Matuszewska et al. PLoS One. .

Abstract

The aim of this study is to investigate in vitro the anticancer, antioxidant, and antibacterial activities of three low molecular weight subfractions I, II and III isolated from secondary metabolites produced by the wood degrading fungus Cerrena unicolor. The present study demonstrated that the low molecular weight subfractions III exhibited the strongest inhibitory activity towards breast carcinoma cells MDA-MB-231, prostatic carcinoma cells PC3, and breast cancer cells MCF7 with the half-maximal inhibitory concentration (IC50) value of 52,25 μg/mL, 60,66 μg/mL, and 54,92 μg/mL, respectively. The highest percentage of inhibition was noted at a concentration of 300 μg/mL in all the examined tumor lines. A significant percentage (59.08%) of ex-LMSIII inhibition of the MDA-MB-231 tumor line was reached at a concentration of 15 μg/ml, while the concentration applied did not affect normal human fibroblast cells. The low molecular weight subfraction III was the most effective and additionally showed the highest free radical 1,1-diphenyl-2-picryl-hydrazyl scavenging activity (IC50 20.39 μg/mL) followed by the low molecular weight subfraction I (IC50 64.14 μg/mL) and II (IC50 49.22 μg/mL). The antibacterial activity of the tested preparations was evaluated against three microorganisms: Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. The MIC minimal inhibitory concentration (MIC) values for the low molecular weight subfraction I, II, and III showed a stronger inhibition effect on S. aureus than on B. subtilis and E. coli cells. The MIC values for the low molecular weight subfraction II against S. aureus, B. subtilis, and E. coli were 6.25, 12.5, and 100 mg/mL, respectively.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1
FT-IR analysis (FT-IR spectra of ex-LMSI (a), ex-LMSII (b), and ex-LMSIII (c) isolated from C. unicolor). FTIR spectroscopy was performed with a spectrometer (Thermo Scientific Nicolet 8700A with FT Raman Nicolet NXR module) in the wavelength range 4000–400 cm−1.
Fig 2
Fig 2. Cytotoxic effect (inhibition activity of ex-LMSI, ex-LMSII, and ex-LMSIII isolated from C. unicolor towards three cancer cell lines MCF7, MDA-MB-23, and PC3 as well as the HSF line).
The MTT assay was performed after 48 h of incubation. Values are mean ± SD (n = 3).
See this image and copyright information in PMC

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