Effects of idebenone (CV-2619) on endogenous monoamine release and cyclic AMP formation in diencephalon slices from rats

Abstract

The addition of CV-2619 (10(-4) M) stimulated the release of endogenous 5-hydroxytryptamine (5-HT) markedly and that of norepinephrine (NE) slightly from slices of rat diencephalon, but it did not affect dopamine (DA) release. The addition of CV-2619 (10(-5)-10(-4) M) or 5-HT (10(-6)-10(-4) M) stimulated cyclic AMP formation in a concentration-dependent manner. Methysergide (10(-4) M), a 5-HT receptor blocker, almost completely blocked CV-2619 (10(-4) M)- and 5-HT (10(-4) M)-induced cyclic AMP formation. These results suggest that CV-2619 stimulates cyclic AMP formation via endogenous monoamine, particularly, 5-HT release.