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. 2018 Mar 5:18:e00245.
doi: 10.1016/j.btre.2018.e00245. eCollection 2018 Jun.

Co-fermentation of the main sugar types from a beechwood organosolv hydrolysate by several strains of Bacillus coagulans results in effective lactic acid production

Robert Glaser  1 , Joachim Venus  1
Affiliations

Co-fermentation of the main sugar types from a beechwood organosolv hydrolysate by several strains of Bacillus coagulans results in effective lactic acid production

Robert Glaser et al. Biotechnol Rep (Amst). .

Erratum in

Abstract

Bacillus coagulans is an interesting facultative anaerobic microorganism for biotechnological production of lactic acid that arouses interest. To determine the efficiency of biotechnological production of lactic acid from lignocellulosic feedstock hydrolysates, five Bacillus coagulans strains were grown in lignocellulose organosolv hydrolysate from ethanol/water-pulped beechwood. Parameter estimation based on a Monod-type model was used to derive the basic key parameters for a performance evaluation of the batch process. Three of the Bacillus coagulans strains, including DSM No. 2314, were able to produce lactate, primarily via uptake of glucose and xylose. Two other strains were identified as having the ability of utilizing cellobiose to a high degree, but they also had a lower affinity to xylose. The lactate yield concentration varied from 79.4 ± 2.1 g/L to 93.7 ± 1.4 g/L (85.4 ± 4.7 % of consumed carbohydrates) from the diluted organosolv hydrolysate.

Keywords: BM, biomass; Biorefinery; CB, cellobiose; Co-fermentation; DSMZ, Leibniz Institute’s German Collection of Microorganisms and Cell Cultures; Glc, glucose; Growth model; LA, lactate; Lactic acid; MO, microorganism; Organosolv; Xyl, xylose.

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Figures

Fig. 1
Fig. 1
Fermentation of organosolv hydrolysate with strain DSM No. 2314. Experimental results are displayed as marks (□ glucose, ◊ xylose, (cellobiose, ○ lactate, + biomass, × (alkaline). Predicted simulation results are shown as lines (− − − glucose, − ▪ − ▪ xylose, — ▪ ▪ cellobiose, − − − lactate, − biomass, ▪ ▪ ▪ alkaline). Diagrams A and B refer to duplicate fermentations with the same inoculum.
Fig. 2
Fig. 2
Fermentation dynamics of organosolv hydrolysate with strain DSM ID 14-301. Experimental results are displayed as marks (□ glucose, ◊ xylose, Δ cellobiose, ○ lactate, + biomass, × alkaline). Predicted simulation results are shown as lines (− − − glucose, − ▪ − ▪ xylose, − ▪ ▪ cellobiose, − − − lactate, − biomass, ▪ ▪ ▪ alkaline). Diagrams A and B refer to duplicate fermentations with the same inoculum.
Fig. 3
Fig. 3
Fermentation dynamics of organosolv hydrolysate with strain DSM ID 14-300. Experimental results are displayed as marks (□ glucose, ◊ xylose, Δ cellobiose, ○ lactate, + biomass, × alkaline). Predicted simulation results are shown as lines (− − − glucose, − ▪ − ▪ xylose, − ▪ ▪ cellobiose, − − − lactate, − biomass, ▪ ▪ ▪ alkaline). Diagrams A and B refer to duplicate fermentations with the same inoculum.
Fig. 4
Fig. 4
Fermentation dynamics of organosolv hydrolysate with strain DSM ID 14-298. Experimental results are displayed as marks (□ glucose, ◊ xylose, Δ cellobiose, ○ lactate, + biomass, × alkaline). Predicted simulation results are shown as lines (− − − glucose, − ▪ − ▪ xylose, − ▪ ▪ cellobiose, − − − lactate, − biomass, ▪ ▪ ▪ alkaline). Diagrams A and B refer to duplicate fermentations with the same inoculum.
Fig. 5
Fig. 5
Fermentation dynamics of organosolv hydrolysate with strain DSM ID 10-395. Experimental results are displayed as marks (□ glucose, ◊ xylose, Δ cellobiose, ○ lactate, + biomass, × alkaline). Predicted simulation results are shown as lines (− − − glucose, − ▪ − ▪ xylose, − ▪ ▪ cellobiose, − − − lactate, − biomass, ▪ ▪ ▪ alkaline). Diagrams A and B refer to duplicate fermentations with the same inoculum.
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