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. 2018 Apr 17:18:e00252.
doi: 10.1016/j.btre.2018.e00252. eCollection 2018 Jun.

Proseek single-plex protein assay kit system to detect sAxl and Gas6 in serological material of brain tumor patients

Heidi Jaksch-Bogensperger  1   2 , Anna Hammerschmid  2 , Ludwig Aigner  2 , Eugen Trinka  3 , Renate Gehwolf  4 , Yvonne Ebner  5 , Markus Hutterer  6 , Sebastien Couillard-Despres  7
Affiliations

Proseek single-plex protein assay kit system to detect sAxl and Gas6 in serological material of brain tumor patients

Heidi Jaksch-Bogensperger et al. Biotechnol Rep (Amst). .

Erratum in

Abstract

•The receptor tyrosine kinase (RTK) Axl and its ligand Gas6 are critically involved in the pathogenesis of high-grade glioma (HGG). Both proteins were found to be overexpressed e.g. in tumor cells, mediating cell proliferation and migration as well as tumor angiogenesis and neuroinflammation. The extracellular domain of Axl (sAxl) and Gas6 were found in the peri-tumoral edema and blood of animals as well as in human glioma tissue. Therefore, we monitored the level of sAxl and Gas6 in human blood samples. To increase the sensitivity of protein detection beyond commonly used standard methods we preliminary tested the innovative Proseek Single-Plex Protein Assay Kit System from Olink Bioscience together with new antibodies against the soluble RTK sAxl and its ligand Gas6. We conclude that the Proseek method is a highly sensitive and fast procedure that can be used as a possible powerful tool compared to routinely used ELISA-methods.

Keywords: Gas6; High-grade glioma (HGG); Proseek; Proximity extension assay (PEA); Serum; sAxl.

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Figures

Fig. 1
Fig. 1
Calibration curve of Gas6 (AF885). Comparison of Proseek (A, B) and DuoSet ELISA Dy885 (C). Detection of each concentration of human recombinant Gas6 from 1 pg/ml to 10 000 pg/ml (n = 3). Bars represent standard deviations of the mean of 3 (A) independent measurements (duplicates) (B). Detection of Gas6 by ELISA Dy885 (C).
Fig. 2
Fig. 2
Calibration curve of Axl (AF154). Comparison of Proseek (A, B) and DuoSet ELISA DY154 (C). Detection of each concentration of human recombinant Axl from 10 pg/ml to 10 000 pg/ml (n = 3). Bars represent standard deviations of the mean of 3 (A) independent measurements (duplicates) (B). Detection of Axl by ELISA DY154 (C).
Fig. 3
Fig. 3
Concentrations of Axl and Gas6 in human serum samples during treatment by using Proseek. The calculations were performed according to the calibration curve in Fig. 1 for Gas6 and Fig. 2 for Axl using the formula y = kx + d. X-axis: P02, P04, P05 serum samples from human patients till recurrence (S). Red dot: serum of a healthy person. y-axis: protein concentration in pg/ml.
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References

    1. Sanai N., Alcarez-Buylla A., Berger M.S. Neural stem cells and the origin of gliomas. New Engl. J. Med. 2005;353:811–822. - PubMed
    1. Louis D.N., Perry A., Reifenberger G. The 2016 World Health Organisation classification of tumors of the central nervous system: a summary. Acta Neuropathol. 2016;131:803–820. - PubMed
    1. Stupp R., Mason W.P., van den Bent M.J. Radiotherapy plus concomitant and aduvant temozolomide for glioblastoma. Engl. J. Med. 2005;352:987–996. - PubMed
    1. Stupp R., Tailibert S., Kesari S. Maintenance therapy with tumor-treating fields plus temozolmide vs temozolomide alone for glioblastoma: a randomized clinical trial. JAMA. 2015;14:2535–2543. - PubMed
    1. Hutterer M., Kzyazev P., Abate A. Axl and growth arrest –specific Gene 6 are frequently overexpressed in human gliomas and predict poor prognosis in patients with glioblastoma multiforme. Clin. Cancer Res. 2008;14:130–138. - PubMed