Hymenobacter segetis sp. nov., isolated from soil

Abstract

A polyphasic taxonomic study was performed on a novel strain designated as S7-3-11^T, which was isolated from soil of the Gyeongsangnam-do province in Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S7-3-11^T belongs to the genus Hymenobacter and is most closely related to Hymenobacter ruber PB156^T (97.9%), Hymenobacter daeguensis 16F3Y-2^T (97.8%), Hymenobacter glaciei VUG-A130^T (97.7%), Hymenobacter soli PB17^T (97.5%), Hymenobacter terrae DG7A^T (97.5%), and Hymenobacter antarcticus VUG-A42aa^T (97.3%). However, DNA-DNA hybridization results showed less than 50% relatedness with respect to the type strains of the six most closely related species. The DNA G + C content of strain S7-3-11^T was 60.2 mol%. MK-7 was identified as the predominant respiratory quinone, and summed feature 3 (C_16:1 ω7c/C_16:1 ω6c; 21.5%), C_15:0 iso (16.8%), C_15:0 anteiso (16.2%), and C_15:1 iso G (10.8%) were the major fatty acids. Phosphatidylethanolamine, an unidentified aminolipid, and an unidentified aminophospholipid were detected as major polar lipids. On the basis of the polyphasic evidence presented, strain S7-3-11^T is considered to represent a novel species of the genus Hymenobacter, for which the name Hymenobacter segetis sp. nov. is proposed. The type strain is S7-3-11^T (= KCTC 52732^T = JCM 32197^T).

Keywords: Bacteroidetes; Hymenobacter; Soil bacteria.