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. 2018 Aug 10;80(8):1236-1240.
doi: 10.1292/jvms.18-0089. Epub 2018 Jun 5.

Detection of felis catus papillomavirus type 3 and 4 DNA from squamous cell carcinoma cases of cats in Japan

Nanako Yamashita-Kawanishi  1 , Ryoma Sawanobori  1 , Kosuke Matsumiya  1 , Akiko Uema  1 , James K Chambers  2 , Kazuyuki Uchida  2 , Hidekatsu Shimakura  1 , Masano Tsuzuki  1 , Chia-Yu Chang  3 , Hui-Wen Chang  3 , Takeshi Haga  1
Affiliations

Detection of felis catus papillomavirus type 3 and 4 DNA from squamous cell carcinoma cases of cats in Japan

Nanako Yamashita-Kawanishi et al. J Vet Med Sci. .

Abstract

Association of felis catus papillomaviruses (FcaPVs) with feline squamous cell carcinoma (SCC) has been reported worldwide, while there is limited information about FcaPVs in Asia. In this study, 21 feline SCC biopsy samples from cats in Japan were analyzed by PCR with PV consensus primers and type-specific primers for FcaPV type 2 (FcaPV-2), FcaPV-3 and FcaPV-4 designed in this study. Sequence analysis revealed that one sample was FcaPV-3, and two were FcaPV-4. In both FcaPV-4 positive samples, 334th tryptophan in L1 ORF was deleted compared with the reference sequence. Moreover, immunohistochemistry showed that p16 protein was positive in both FcaPV-4 detected samples. This study would contribute to the molecular epidemiological and pathological understanding of FcaPV in Japan.

Keywords: FcaPV type 3; FcaPV type 4; Feline squamous cell carcinoma; Japan; type specific primer.

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Figures

Fig. 1.
Fig. 1.
Representative histopathological findings of feline squamous cell carcinoma. Atypical squamous cells invade the fibrous tissue. (A) Case number 14-1110, FcaPV4-positive. (B) Case number 13-0153, FcaPV3-positive. (C) Case number 14-0778, FcaPV negative. Bars, 100 µm.
Fig. 2.
Fig. 2.
Immunohistochemical analysis against p16 protein in feline squamous cell carcinoma. (A) Intense positive p16 staining in the basal cell membrane (FcaPV4-positive, case number 14-1110). (B) Negative for p16 (FcaPV-negative, case number 14-0778). Bars, 100 μm.
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