Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Apr 11;13(7):2251-65.
doi: 10.1093/nar/13.7.2251.

Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region

S Moriya, N Ogasawara, H Yoshikawa
Free PMC article

Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region

S Moriya et al. Nucleic Acids Res. .
Free PMC article

Abstract

Approximately 10,000 nucleotides were sequenced in the oriC region of the Bacillus subtilis chromosome. The first replicating DNA strands are hybridized with a SalI-EcoRI fragment (nucleotide #1206-2954) in one direction (left to right) and an EcoRI-PstI fragment (#2949-4233) in the other. Seven open reading frames (ORF) accompanied with Shine-Dalgarno (SD) sequences were identified. ORF638 and ORF821 were identified as gyrB and gyrA genes respectively based on genetic evidences and amino acid sequence data. Comparison of amino acid sequences revealed that ORF44, ORF446, ORF378 and ORF323 are homologous with rpmH, dnaA, dnaN and recF of Escherichia coli, respectively. Thus, the organization of the ORFs from ORF44 to ORF638 resembles the organization of genes in the rpmH-gyrB region of the E. coli chromosome. Two non-coding regions characteristic for oriC signals were found near the site of initiation of the first replicating DNA. They are composed of repeating sequences whose consensus sequence TTAT(C/A)CACA is identical to that of 4 repeating sequences in the oriC of E. coli.

PubMed Disclaimer

References

    1. Mol Gen Genet. 1980 Apr;178(1):9-20 - PubMed
    1. J Bacteriol. 1982 Jul;151(1):524-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4285-9 - PubMed
    1. Nucleic Acids Res. 1982 Nov 25;10(22):7373-85 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1164-8 - PubMed

Publication types

Associated data

LinkOut - more resources