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. 1985 May 10;13(9):3043-62.
doi: 10.1093/nar/13.9.3043.

Cloning and characterization of the DAS gene encoding the major methanol assimilatory enzyme from the methylotrophic yeast Hansenula polymorpha

Z A Janowicz, M R Eckart, C Drewke, R O Roggenkamp, C P Hollenberg, J Maat, A M Ledeboer, C Visser, C T Verrips
Free PMC article

Cloning and characterization of the DAS gene encoding the major methanol assimilatory enzyme from the methylotrophic yeast Hansenula polymorpha

Z A Janowicz et al. Nucleic Acids Res. .
Free PMC article

Abstract

A gene library from the methanol utilizing yeast Hansenula polymorpha, constructed in a lambda Charon4A vector, was used to clone the gene encoding a key methanol assimilating enzyme, dihydroxyacetone synthase (DHAS) by differential plaque hybridization. The nucleotide sequence of the 2106 bp structural gene and the 5' and 3' non-coding regions was determined. The deduced amino acid sequence of the protein is in agreement with the apparent molecular weight and amino acid composition of the purified protein. The codon bias is not so pronounced as in some Saccharomyces cerevisiae genes.

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References

    1. J Biol Chem. 1977 Feb 10;252(3):1102-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 - PubMed
    1. Ann N Y Acad Sci. 1982;386:200-16 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 - PubMed
    1. Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 - PubMed

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