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. 1985 May;82(10):3335-9.
doi: 10.1073/pnas.82.10.3335.

A systematic study of HLA class II-beta DNA restriction fragments in insulin-dependent diabetes mellitus

O Cohen-Haguenauer, E Robbins, C Massart, M Busson, I Deschamps, J Hors, J M Lalouel, J Dausset, D Cohen

A systematic study of HLA class II-beta DNA restriction fragments in insulin-dependent diabetes mellitus

O Cohen-Haguenauer et al. Proc Natl Acad Sci U S A. 1985 May.

Abstract

DNA restriction fragments of the genes encoding HLA class II-beta antigens were compared in 34 patients with insulin-dependent diabetes mellitus and 34 HLA-DR-matched healthy individuals. Ninety-three fragments, determined by six restriction enzymes (EcoRI, EcoRV, HindIII, BamHI, Pvu II, and Taq I), were analyzed: (i) A DR Taq I 12.7-kilobase-pair fragment might be a marker for the extended haplotype HLA-B8, DR3. (ii) In controls, DR4 haplotypes are associated with two distinct clusters of DQ restriction fragments (DQR4 and DQR5). Almost all (94%) DR4 patients belong to the DQR4 and not to the DQR5 cluster. This suggests that, among HLA-DR4 haplotypes, only DQR4 haplotypes are involved in susceptibility to insulin-dependent diabetes mellitus. (iii) A DR Taq I 14.5-kilobase-pair fragment was found to be strongly associated with DQR4, mainly in DR3/DR4 heterozygous patients (P = 5 X 10(-4). However, these results must be interpreted with caution, taking into account the high number of statistical tests performed.

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