Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 May-Jun;8(3):246-253.

In vitro evaluation of antibacterial activity of verbascoside, lemon verbena extract and caffeine in combination with gentamicin against drug-resistant Staphylococcus aureus and Escherichia coli clinical isolates

Affiliations

In vitro evaluation of antibacterial activity of verbascoside, lemon verbena extract and caffeine in combination with gentamicin against drug-resistant Staphylococcus aureus and Escherichia coli clinical isolates

Bibi Sedigheh Fazly Bazzaz et al. Avicenna J Phytomed. 2018 May-Jun.

Abstract

Objective: In recent years, there has been an increasing interest in using herbal products to overcome bacterial resistance. The aim of this study was to investigate the effect of lemon verbena aqueous extract, verbascoside and caffeine in combination with gentamicin against standard and clinical isolates of Staphylococcus aureus and Escherichia coli strains.

Materials and methods: The MIC and MBC values of different antibacterial agents against bacterial strains were determined. The effect of co-administration lemon verbena extract, verbascoside, and caffeine and gentamicin was studied in vitro using a checkerboard method and calculating fraction inhibitory concentration index (FICI).

Results: Herbal extract, verbascoside and caffeine alone showed no inhibitory effects on any of the bacterial strains (at doses up to 200 g/ml). Herbal extract, verbascoside and caffeine were able to decrease the MIC of gentamicin against the standard resistant strains and two clinical isolates. Among these combinations, the co-administration of verbascoside and gentamicin was more effective and synergistic activities (FICI<1)‏ against clinical isolates were observed.‏.

Conclusion: The results of the present study revealed that herbal extract, verbascoside and caffeine potentiated the antimicrobial action of gentamicin against some clinical isolates of S. aureus and E. coli.

Keywords: Antibiotics; Combination therapy; Herbal extract; Methylxanthines‎; Natural compounds; Resistant pathogens.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Akaberi M, Iranshahy M, Iranshahi M. Review of the traditional uses, phytochemistry pharmacology and toxicology of giant fennel (Ferula communis l subsp communis) Iran J Basic Med Sci. 2015;18:1050–1062. - PMC - PubMed
    1. Alipieva K, Korkina L, Orhan IE, Georgiev MI. Verbascoside — A review of its occurrence, (bio) synthesis and pharmacological significance. Biotechnol Adv. 2014;32:1065–1076. - PubMed
    1. Azimi H, Fallah-Tafti M, Khakshur AA, Abdollahi M. A review of phytotherapy of acne vulgaris: perspective of new pharmacological treatments. Fitoterapia. 2012;83:1306–17. - PubMed
    1. Bazzaz BS, Lavaei S, Hosseinzadeh H. Interaction of methylxanthines and gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa: role of phosphodiesterase inhibition. Acta Microbiol Immunol Hung. 2012;59:13–20. - PubMed
    1. Carnat A, Carnat AP, Fraisse D, Lamaison JL. The aromatic and polyphenolic composition of lemon verbena tea. Fitoterapia. 1999;70:44–49. - PubMed

LinkOut - more resources