In vitro evaluation of antibacterial activity of verbascoside, lemon verbena extract and caffeine in combination with gentamicin against drug-resistant Staphylococcus aureus and Escherichia coli clinical isolates
- PMID: 29881710
- PMCID: PMC5987439
In vitro evaluation of antibacterial activity of verbascoside, lemon verbena extract and caffeine in combination with gentamicin against drug-resistant Staphylococcus aureus and Escherichia coli clinical isolates
Abstract
Objective: In recent years, there has been an increasing interest in using herbal products to overcome bacterial resistance. The aim of this study was to investigate the effect of lemon verbena aqueous extract, verbascoside and caffeine in combination with gentamicin against standard and clinical isolates of Staphylococcus aureus and Escherichia coli strains.
Materials and methods: The MIC and MBC values of different antibacterial agents against bacterial strains were determined. The effect of co-administration lemon verbena extract, verbascoside, and caffeine and gentamicin was studied in vitro using a checkerboard method and calculating fraction inhibitory concentration index (FICI).
Results: Herbal extract, verbascoside and caffeine alone showed no inhibitory effects on any of the bacterial strains (at doses up to 200 g/ml). Herbal extract, verbascoside and caffeine were able to decrease the MIC of gentamicin against the standard resistant strains and two clinical isolates. Among these combinations, the co-administration of verbascoside and gentamicin was more effective and synergistic activities (FICI<1) against clinical isolates were observed..
Conclusion: The results of the present study revealed that herbal extract, verbascoside and caffeine potentiated the antimicrobial action of gentamicin against some clinical isolates of S. aureus and E. coli.
Keywords: Antibiotics; Combination therapy; Herbal extract; Methylxanthines; Natural compounds; Resistant pathogens.
Similar articles
-
Effect of Catechins, Green tea Extract and Methylxanthines in Combination with Gentamicin Against Staphylococcus aureus and Pseudomonas aeruginosa: - Combination therapy against resistant bacteria.J Pharmacopuncture. 2016 Dec;19(4):312-318. doi: 10.3831/KPI.2016.19.032. J Pharmacopuncture. 2016. PMID: 28097041 Free PMC article.
-
Effects of Omeprazole and Caffeine Alone and in Combination with Gentamicin and Ciprofloxacin Against Antibiotic Resistant Staphylococcus Aureus and Escherichia Coli Strains.J Pharmacopuncture. 2019 Mar;22(1):49-54. doi: 10.3831/KPI.2019.22.006. Epub 2019 Mar 31. J Pharmacopuncture. 2019. PMID: 30989001 Free PMC article.
-
Synergistic antibacterial effects of herbal extracts and antibiotics on methicillin-resistant Staphylococcus aureus: A computational and experimental study.Exp Biol Med (Maywood). 2017 Apr;242(7):731-743. doi: 10.1177/1535370216689828. Epub 2017 Jan 1. Exp Biol Med (Maywood). 2017. PMID: 28118725 Free PMC article.
-
An experimental study to evaluate the effect of polymixin E (Colistin) alone or in combination with gentamicin in McCarey-Kaufman corneal preservation medium on various drug resistant bacterial and fungal isolates.Indian J Ophthalmol. 2022 Aug;70(8):2950-2955. doi: 10.4103/ijo.IJO_12_22. Indian J Ophthalmol. 2022. PMID: 35918951 Free PMC article.
-
Cleistochlamys kirkii chemical constituents: Antibacterial activity and synergistic effects against resistant Staphylococcus aureus strains.J Ethnopharmacol. 2016 Feb 3;178:180-7. doi: 10.1016/j.jep.2015.12.009. Epub 2015 Dec 8. J Ethnopharmacol. 2016. PMID: 26674158
Cited by
-
The association between dietary phytochemical index and bacterial vaginosis risk: secondary analysis of case-control study.J Health Popul Nutr. 2024 Aug 31;43(1):135. doi: 10.1186/s41043-024-00631-2. J Health Popul Nutr. 2024. PMID: 39217390 Free PMC article.
-
Research Progress and Trends of Phenylethanoid Glycoside Delivery Systems.Foods. 2022 Mar 7;11(5):769. doi: 10.3390/foods11050769. Foods. 2022. PMID: 35267401 Free PMC article. Review.
-
Clinically relevant materials & applications inspired by food technologies.EBioMedicine. 2022 Jan;75:103792. doi: 10.1016/j.ebiom.2021.103792. Epub 2021 Dec 30. EBioMedicine. 2022. PMID: 34974308 Free PMC article. Review.
-
Towards Advances in Medicinal Plant Antimicrobial Activity: A Review Study on Challenges and Future Perspectives.Microorganisms. 2021 Sep 27;9(10):2041. doi: 10.3390/microorganisms9102041. Microorganisms. 2021. PMID: 34683362 Free PMC article. Review.
-
Chemometric Profiling and Bioactivity of Verbena (Aloysia citrodora) Methanolic Extract from Four Localities in Tunisia.Foods. 2021 Nov 24;10(12):2912. doi: 10.3390/foods10122912. Foods. 2021. PMID: 34945462 Free PMC article.
References
-
- Alipieva K, Korkina L, Orhan IE, Georgiev MI. Verbascoside — A review of its occurrence, (bio) synthesis and pharmacological significance. Biotechnol Adv. 2014;32:1065–1076. - PubMed
-
- Azimi H, Fallah-Tafti M, Khakshur AA, Abdollahi M. A review of phytotherapy of acne vulgaris: perspective of new pharmacological treatments. Fitoterapia. 2012;83:1306–17. - PubMed
-
- Bazzaz BS, Lavaei S, Hosseinzadeh H. Interaction of methylxanthines and gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa: role of phosphodiesterase inhibition. Acta Microbiol Immunol Hung. 2012;59:13–20. - PubMed
-
- Carnat A, Carnat AP, Fraisse D, Lamaison JL. The aromatic and polyphenolic composition of lemon verbena tea. Fitoterapia. 1999;70:44–49. - PubMed
LinkOut - more resources
Full Text Sources
Molecular Biology Databases