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. 1985;34(2-3):243-51.
doi: 10.1016/0378-1119(85)90133-7.

Nucleotide sequence coding for the insecticidal fragment of the Bacillus thuringiensis crystal protein

Y Shibano, A Yamagata, N Nakamura, T Iizuka, H Sugisaki, M Takanami

Nucleotide sequence coding for the insecticidal fragment of the Bacillus thuringiensis crystal protein

Y Shibano et al. Gene. 1985.

Abstract

The insecticidal crystal protein (ICP) gene, icp, from a 68-kb plasmid derived from Bacillus thuringiensis subsp. sotto was cloned in Escherichia coli. The icp expression in E. coli cells was confirmed by both immunological and insect-toxicity assays of the cell extract. The entire icp gene resides in the 6.6-kb PstI fragment, which codes for a 144-kDal peptide identical to the intact ICP, as determined by its size and reaction with anti-ICP antibody. Deletion analysis further revealed that the 2.8-kb region within the 6.6-kb PstI fragment codes for ICP. Analysis of the nucleotide sequence indicated that a peptide of 934 amino acid residues truncated at the C-terminal end is encoded by this 2.8-kb fragment. A unique feature of this truncated ICP is the abundance of cysteine and lysine residues within its C-terminal region.

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