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Review
. 2018 Jun 11;8(2):54.
doi: 10.3390/bios8020054.

Aptamer-Based Biosensors for Antibiotic Detection: A Review

Asol Mehlhorn  1 , Parvaneh Rahimi  2 , Yvonne Joseph  3
Affiliations
Review

Aptamer-Based Biosensors for Antibiotic Detection: A Review

Asol Mehlhorn et al. Biosensors (Basel). .

Abstract

Antibiotic resistance and, accordingly, their pollution because of uncontrolled usage has emerged as a serious problem in recent years. Hence, there is an increased demand to develop robust, easy, and sensitive methods for rapid evaluation of antibiotics and their residues. Among different analytical methods, the aptamer-based biosensors (aptasensors) have attracted considerable attention because of good selectivity, specificity, and sensitivity. This review gives an overview about recently-developed aptasensors for antibiotic detection. The use of various aptamer assays to determine different groups of antibiotics, like β-lactams, aminoglycosides, anthracyclines, chloramphenicol, (fluoro)quinolones, lincosamide, tetracyclines, and sulfonamides are presented in this paper.

Keywords: ampicillin; antibiotic; aptamer; aptasensor; biosensor; chloramphenicol; ciprofloxacin; danofloxacin; daunomycin; enrofloxacin; gentamicin; kanamycin; lincomycin; neomycin; ofloxacin; oxytetracycline; penicillin; streptomycin; sulfadimethoxine; tetracycline; tobramycin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Working principles of the most widely used aptasensors. (a) Quartz crystal microbalance; (b) surface acoustic wave; (c) micromechanical cantilever array; (d) AuNPs based colorimetric aptasensor; (e) fluorometric aptasensor; (f) surface plasmon resonance; (g) surface enhanced Raman scattering; and (h) electrochemical aptasensor.
Figure 2
Figure 2
Chemical structure of: (a) ampicillin and (b) penicillin G. The β-lactam ring is marked in red.
Figure 3
Figure 3
Chemical structure of: (a) gentamicin; (b) kanamycin; (c) neomycin B; (d) tobramycin; and (e) streptomycin. The basic structure of aminoglycoside antibiotics consists of an aminocyclitol ring (marked in red) which is linked glycosidically to other amino sugars.
Figure 4
Figure 4
Chemical structure of daunomycin. The basic structure of the anthracyclines is marked in red.
Figure 5
Figure 5
Chemical structure of chloramphenicol.
Figure 6
Figure 6
Chemical structure of: (a) ciprofloxacin; (b) danofloxacin; (c) enrofloxacin; and (d) ofloxacin. The structure of quinolone is marked in red.
Figure 7
Figure 7
Chemical structure of lincomycin. The basic structure of the anthracyclines is marked in red.
Figure 8
Figure 8
Chemical structure of: (a) oxytetracycline and (b) tetracycline. The basic structure of the tetracyclines is marked in red.
Figure 9
Figure 9
Chemical structure of sulfadimethoxine. The sulfonamide group is marked in red.
See this image and copyright information in PMC

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