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. 1985 Jul;163(1):275-81.
doi: 10.1128/jb.163.1.275-281.1985.

Cloning and mapping of the genetic determinants for microcin B17 production and immunity

J L San Millan, C Hernandez-Chico, P Pereda, F Moreno

Cloning and mapping of the genetic determinants for microcin B17 production and immunity

J L San Millan et al. J Bacteriol. 1985 Jul.

Abstract

Plasmid pMccB17 (70 kilobases [kb]) codes for the production of microcin B17, a peptide that inhibits DNA synthesis, and for microcin B17 immunity. A BamHI-EcoRI fragment of 5.1 kb from pMccB17 was cloned into pBR322 in two steps. The resulting plasmid (pMM102) overproduced microcin B17 and expressed immunity against microcin. Mcc- and Mcc- Imm- mutants were isolated on plasmids pMccB17 and pMM102 by deleting various DNA fragments and by inserting different translocatable elements. Physical and phenotypic characterization of these mutants showed that a DNA region of 3.0 to 3.5 kb is required to produce microcin B17, whereas an adjacent region of about 1.0 kb is required to express microcin B17 immunity.

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