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. 1985 Jul;163(1):398-400.
doi: 10.1128/jb.163.1.398-400.1985.

Cloning of the contiguous 165-kilobase-pair region around the terminus of Escherichia coli K-12 DNA replication

K Asada, S Nakatani, M Takanami

Cloning of the contiguous 165-kilobase-pair region around the terminus of Escherichia coli K-12 DNA replication

K Asada et al. J Bacteriol. 1985 Jul.

Abstract

Escherichia coli K-12 chromosomal DNA was partially digested with either EcoRI or HindIII, and cosmid libraries were constructed. By screening these libraries, a series of partially overlapping clones which covered the terC region was isolated. The cloned area spanned about 165 kilobase pairs, corresponding to the 29.7-to-33.2-min region of the genetic map of the E. coli chromosome.

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