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. 2018 Apr 5;53(3):287-292.
doi: 10.1016/j.rboe.2018.03.017. eCollection 2018 May-Jun.

Effects of simvastatin associated with exercise on the mechanical resistance of muscle and bone in rats

Jéssica Suzuki Yamanaka  1 , Kaique Eduardo Carvalho Ribeiro  2 , Gabriela Rezende Yanagihara  1 , Antônio Carlos Shimano  1 , Álvaro César de Oliveira Penoni  3
Affiliations

Effects of simvastatin associated with exercise on the mechanical resistance of muscle and bone in rats

Jéssica Suzuki Yamanaka et al. Rev Bras Ortop. .

Abstract
in English, Portuguese

Objective: The aim of the present study was to evaluate the influence of simvastatin on mechanical properties of muscle and bone in hypercholesterolemic rats submitted to physical exercise.

Methods: Ten male Wistar rats were submitted to a high-fat diet rich in cholesterol for 90 days. The animals were then divided into two groups: animals treated with physical exercise (EG) and animals treated with physical exercise and simvastatin (ESG). Protocols for physical exercise in water and simvastatin administration were performed for eight weeks. After this period, the animals were euthanized; the left tibia and right gastrocnemius muscle were dissected for mechanical analysis, and the right tibia for densitometry. The data were analyzed using Student's t-test, considering a level of significance of 5%.

Results: The comparison of maximum load and stiffness revealed no significant differences between the groups for both the tibia (p = 0.851 and p = 0.259) and the gastrocnemius (p = 0.911 and p = 0.083). The tibia BMD also showed no significant difference between the groups (p = 0.803).

Conclusion: Simvastatin had no negative effects on mechanical properties in tibia and gastrocnemius of hypercholesterolemic rats submitted to physical exercise.

Objetivo: Avaliar a influência da sinvastatina nas propriedades mecânicas de ossos e músculos de ratos hipercolesterolêmicos submetidos a exercício físico.

Métodos: Foram usados dez ratos machos da raça Wistar, submetidos a dieta hiperlipídica rica em colesterol por 90 dias. Os animais foram então distribuídos em dois grupos: submetidos a tratamento com exercício físico (GE) e submetidos a tratamento com exercício físico e sinvastatina (GE + S). Foi aplicado um protocolo de exercício físico na água e administração de sinvastatina por oito semanas. Após esse período, os animais foram eutanasiados e dissecados a tíbia esquerda e o músculo gastrocnêmio direito de cada animal para análise mecânica e a tíbia direita para densitometria. Para análise dos dados foi aplicado o teste t de Student, considerou-se nível de significância de 5%.

Resultados: A comparação da força máxima e rigidez não revelou diferença significativa entre os grupos tanto para a tíbia (p = 0,851 e p = 0,259) quanto para o músculo gastrocnêmio (p = 0,911 e p = 0,083). A DMO das tíbias também não apresentou diferença significativa entre os grupos (p = 0,803).

Conclusão: A sinvastatina não teve efeitos deletérios nas propriedades mecânicas da tíbia e do músculo gastrocnêmio de ratos hipercolesterolêmicos submetidos a exercício físico aeróbio.

Keywords: Exercise; Gastrocnemius; Hypercholesterolemia; Simvastatin; Tibia.

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Figures

Fig. 1
Fig. 1
(A) Comparison between exercise (EG) and exercise + simvastatin (ESG) groups at three different times. Both groups presented weight gain until the end of the diet and had weight loss with treatment, with no significant difference between groups at any of the times. *Significant difference in relation to the beginning. +Significant difference in relation to post-treatment; (B) feed intake did not change before and after treatment and did not differ between groups.
Fig. 2
Fig. 2
(A) Exercise (EG) and exercise and simvastatin (ESG) groups presented similar maximum strength at the mechanical gastrocnemius test; (B) rigidity was similar between groups.
See this image and copyright information in PMC

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