Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Aug;99(2):451-457.
doi: 10.4269/ajtmh.18-0201. Epub 2018 Jun 7.

Genetic Characterization of the Patois Serogroup (Genus Orthobunyavirus; Family Peribunyaviridae) and Evidence That Estero Real Virus is a Member of the Genus Orthonairovirus

Patricia V Aguilar  1   2   3 , William Marciel de Souza  4 , Jesus A Silvas  2   3   1 , Thomas Wood  5 , Steven Widen  5 , Marcílio Jorge Fumagalli  4 , Márcio Roberto Teixeira Nunes  6
Affiliations

Genetic Characterization of the Patois Serogroup (Genus Orthobunyavirus; Family Peribunyaviridae) and Evidence That Estero Real Virus is a Member of the Genus Orthonairovirus

Patricia V Aguilar et al. Am J Trop Med Hyg. 2018 Aug.

Abstract

Estero Real virus (ERV) was isolated in 1980 from Ornithodoros tadaridae ticks collected in El Estero Real, Sancti Spiritus, Cuba. Antigenic characterization of the isolate based on serological methods found a relationship with Abras and Zegla viruses and, consequently, the virus was classified taxonomically within the Patois serogroup. Given the fact that genetic characterization of Patois serogroup viruses has not yet been reported and that ERV is the only virus within the Patois serogroup isolated from ticks, we recently conducted nearly complete genome sequencing in an attempt to gain further insight into the genetic relationship of ERV with other Patois serogroup viruses and members of Peribunyaviridae family (Bunyavirales order). With the exception of ERV, our sequencing and phylogenetic studies revealed the close relationship of the Patois serogroup viruses to each other, forming a clear divergent clade from other members of the Orthobunyavirus genus (Peribunyaviridae family). Notably, our analysis also revealed that ERV forms a monophyletic clade that is closely related to species of the Orthonairovirus genus (Nairoviridae family) in all the genome segments. In light of these findings, we believe that the taxonomic classification of ERV should be revised.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Genome organization of Patois serogroup. (A) Abras virus, (B) Babahoyo virus, (C) Patois virus, (D) Shark River virus, (E) Zegla virus, and (F) Estero Real virus. This figure appears in color at www.ajtmh.org.
Figure 2.
Figure 2.
Maximum likelihood phylogenetic trees of Patois serogroup within Orthobunyavirus genus. The nucleoprotein (A), glycoprotein (B), and RdRP (C), all based on LG + I + G4 model amino acids substitution model. Phylogenies are midpoint rooted and with proportional branches for clarity of presentation. The scale bar indicates evolutionary distance in numbers of substitutions per amino acid substitutions/site, and the principal bootstrap support levels were indicated. The Patois virus strains sequenced in this study are highlighted in light grey and in red (online). This figure appears in color at www.ajtmh.org.
Figure 3.
Figure 3.
Pairwise genetic similarities (amino acid p-distance) of Patois serogroup compared with other orthobunyavirus groups based on the coding sequencing of N protein (A), glycopolyprotein (B), and RdRp (C). This figure appears in color at www.ajtmh.org.
Figure 4.
Figure 4.
Maximum likelihood phylogenetic trees of ERV within Nairoviridae family. The nucleoprotein (A) and glycoprotein (B) based on LG + I + G4 model amino acids substitution model and RdRP (C) based on LG + F + I + G4 model amino acids substitution model. Phylogenies are midpoint rooted and with proportional branches for clarity of presentation. The scale bar indicates evolutionary distance in numbers of substitutions per amino acids substitutions/site, and the principal bootstrap support levels were indicated. Estero Real virus sequenced in this study is highlighted in light grey and in red (online). This figure appears in color at www.ajtmh.org.
Figure 5.
Figure 5.
Pairwise genetic similarities (amino acid p-distance) of ERV compared with other orthonairovirus groups based on the coding sequencing of N protein (A), glycopolyprotein (B), and RdRp (C). This figure appears in color at www.ajtmh.org.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

  • Taxonomy of the order Bunyavirales: update 2019.
    Abudurexiti A, Adkins S, Alioto D, Alkhovsky SV, Avšič-Županc T, Ballinger MJ, Bente DA, Beer M, Bergeron É, Blair CD, Briese T, Buchmeier MJ, Burt FJ, Calisher CH, Cháng C, Charrel RN, Choi IR, Clegg JCS, de la Torre JC, de Lamballerie X, Dèng F, Di Serio F, Digiaro M, Drebot MA, Duàn X, Ebihara H, Elbeaino T, Ergünay K, Fulhorst CF, Garrison AR, Gāo GF, Gonzalez JJ, Groschup MH, Günther S, Haenni AL, Hall RA, Hepojoki J, Hewson R, Hú Z, Hughes HR, Jonson MG, Junglen S, Klempa B, Klingström J, Kòu C, Laenen L, Lambert AJ, Langevin SA, Liu D, Lukashevich IS, Luò T, Lǚ C, Maes P, de Souza WM, Marklewitz M, Martelli GP, Matsuno K, Mielke-Ehret N, Minutolo M, Mirazimi A, Moming A, Mühlbach HP, Naidu R, Navarro B, Nunes MRT, Palacios G, Papa A, Pauvolid-Corrêa A, Pawęska JT, Qiáo J, Radoshitzky SR, Resende RO, Romanowski V, Sall AA, Salvato MS, Sasaya T, Shěn S, Shí X, Shirako Y, Simmonds P, Sironi M, Song JW, Spengler JR, Stenglein MD, Sū Z, Sūn S, Táng S, Turina M, Wáng B, Wáng C, Wáng H, Wáng J, Wèi T, Whitfield AE, Zerbini FM, Zhāng J, Zhāng L, Zhāng Y, Zhang YZ, Zhāng Y, Zhou X, Zhū L, Kuhn JH. Abudurexiti A, et al. Arch Virol. 2019 Jul;164(7):1949-1965. doi: 10.1007/s00705-019-04253-6. Epub 2019 May 7. Arch Virol. 2019. PMID: 31065850 Free PMC article.
  • Genomic characterization of 99 viruses from the bunyavirus families Nairoviridae, Peribunyaviridae, and Phenuiviridae, including 35 previously unsequenced viruses.
    Kapuscinski ML, Bergren NA, Russell BJ, Lee JS, Borland EM, Hartman DA, King DC, Hughes HR, Burkhalter KL, Kading RC, Stenglein MD. Kapuscinski ML, et al. PLoS Pathog. 2021 Mar 1;17(3):e1009315. doi: 10.1371/journal.ppat.1009315. eCollection 2021 Mar. PLoS Pathog. 2021. PMID: 33647063 Free PMC article.
  • Genetic Characterization of Frijoles and Chilibre Species Complex Viruses (Genus Phlebovirus; Family Phenuiviridae) and Three Unclassified New World Phleboviruses.
    Hughes HR, Russell BJ, Lambert AJ. Hughes HR, et al. Am J Trop Med Hyg. 2020 Feb;102(2):359-365. doi: 10.4269/ajtmh.19-0717. Am J Trop Med Hyg. 2020. PMID: 31802735 Free PMC article.
  • Barrita Virus, a Novel Virus of the Patois Serogroup (Genus Orthobunyavirus; Family Peribunyaviridae).
    Fernández D, Marciel de Souza W, Silvas JA, Deardorff ER, Widen SG, Estrada-Franco JG, Weaver SC, Nunes M, Aguilar PV. Fernández D, et al. Am J Trop Med Hyg. 2020 Jul;103(1):190-192. doi: 10.4269/ajtmh.19-0906. Epub 2020 May 21. Am J Trop Med Hyg. 2020. PMID: 32458789 Free PMC article.
  • Human-Pathogenic Kasokero Virus in Field-Collected Ticks.
    Schuh AJ, Amman BR, Patel K, Sealy TK, Swanepoel R, Towner JS. Schuh AJ, et al. Emerg Infect Dis. 2020 Dec;26(12):2944-2950. doi: 10.3201/eid2612.202411. Emerg Infect Dis. 2020. PMID: 33219649 Free PMC article.

References

    1. Adams MJ, et al. 2017. Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2017). Arch Virol 162: 2505–2538. - PubMed
    1. Calisher CH, 1996. Chapter 1, Elliot RM, ed. The Bunyaviridae. New York, NY: Springer 1–17.
    1. Scherer WF, Dickerman RW, Ordonez JV, Seymour C, Kramer LD, Jahrling PB, Powers CD, 1976. Ecologic studies of Venezuelan encephalitis virus and isolations of Nepuyo and Patois viruses during 1968–1973 at a marsh habitat near the epicenter of the 1969 outbreak in Guatemala. Am J Trop Med Hyg 25: 151–162. - PubMed
    1. Scherer WF, Dickerman RW, Cupp EW, Ordonez JV, 1985. Ecologic observations of Venezuelan encephalitis virus in vertebrates and isolations of Nepuyo and Patois viruses from sentinel hamsters at Pacific and Atlantic habitats in Guatemala, 1968–1980. Am J Trop Med Hyg 34: 790–798. - PubMed
    1. Srihongse S, Galindo P, Grayson MA, 1966. Isolation of group C arboviruses in Panama including two new members, Patois and Zegla. Am J Trop Med Hyg 15: 379–384. - PubMed

Publication types

LinkOut - more resources