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. 1985 Jun;21(6):969-71.
doi: 10.1128/jcm.21.6.969-971.1985.

Rapid neutralization assay for human cytomegalovirus antibody

M Zerbini, M Plazzi, M Musiani

Rapid neutralization assay for human cytomegalovirus antibody

M Zerbini et al. J Clin Microbiol. 1985 Jun.

Abstract

Human cytomegalovirus induces the appearance of immediate early antigens in infected cells 1 h after infection. This provided the basis for the development of a rapid neutralization assay for cytomegalovirus antibody which was able to yield results within a single day. Indirect immunofluorescence to visualize immediate early antigen-positive cells was applied to the rapid determination of cytomegalovirus-neutralizing antibody. The neutralization titers obtained with this assay on 92 serum samples were in accordance with the immune status as determined by enzyme-linked immunosorbent assay to cytomegalovirus-induced immediate early, early, and late antigens.

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