Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Apr 25;9(2):195-203.
doi: 10.1007/s13167-018-0135-9. eCollection 2018 Jun.

Pathology-specific molecular profiles of saliva in patients with multiple dental caries-potential application for predictive, preventive and personalised medical services

Pavel Seredin  1 , Dmitry Goloshchapov  1 , Yuri Ippolitov  2 , Pimm Vongsvivut  3
Affiliations

Pathology-specific molecular profiles of saliva in patients with multiple dental caries-potential application for predictive, preventive and personalised medical services

Pavel Seredin et al. EPMA J. .

Abstract

Background: Improving the quality of life is part of the global agenda. The focus is predominantly on prevention of socially significant diseases. Combating dental caries-related diseases is a top priority as it has a huge impact on people's social lives. Therefore, the purpose of the work was to study the changes in the molecular composition of saliva from subjects with multiple caries lesions using spectroscopic methods of analysis to identify potential tissue markers of caries development for predictive, preventive and personalised medical services.

Objectives and methods: The molecular composition of mixed saliva (oral fluid) from subjects with and without multiple caries was analysed with the use of spectroscopic techniques, FTIR with synchrotron radiation for the excitation. The IR spectra of the oral fluid as well as the calculated mineral-organic, carbon-phosphate, Amide II/Amide I and protein/thiocyanate ratios were compared between subjects with and without multiple caries.

Results: This complex analysis of the obtained experimental data determined that the molecular composition of the oral fluid from those with multiple caries differed from those without caries; the organic-mineral balance in the oral fluid of those with multiple caries shifted towards a reduction in the mineral complexes, accompanied by an increase in the organic component. The thiocyanate content increased more than twofold, accompanied by increased carboxyl groups of esters, lipids and carbohydrates.

Conclusion: The detected features in the IR spectra of mixed saliva as well as the calculated changes in the ratios between organic and inorganic components can be used as biomarkers of cariogenesis in the oral cavity, as a diagnostic criterion in the analysis of the oral fluid samples.

Keywords: Biomarkers; FTIR spectroscopy; Molecular composition; Personalised medical service; Predictive; Preventive; Thiocyanate level.

PubMed Disclaimer

Conflict of interest statement

Compliance with ethical standardsThe authors declare that they have no competing interests.Not applicable.All patients whose data were used within the created survey had signed institutional consent for the participation research. All persons who participated in the survey signed written consent. Voronezh State University Ethics Committee approved this study (approval number 001.018-2017). The study was carried out in accordance with the approved guidelines.

Figures

Fig. 1
Fig. 1
IR spectra of oral fluid samples averaged over the study groups: with (curve 1) and without (curve 2) multiple caries
Fig. 2
Fig. 2
Calculated R1-R4 ratios obtained for the two groups of participants at examination. a Mineral-organic ratio (R1). b Carbon-phosphate ratio (R2). c Amide II/Amide I ratio (R3). d Protein/thiocyanate ratio (R4)
Fig. 3
Fig. 3
Scheme of the experiment and the most indicative changes in the composition of the oral fluid of those with multiple caries occurred in relation to the number of −N=C=S groups associated with the presence of thiocyanate
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Golubnitschaja O, Baban B, Boniolo G, Wang W, Bubnov R, Kapalla M, Krapfenbauer K, Mozaffari MS, Costigliola V. Medicine in the early twenty-first century: paradigm and anticipation—EPMA position paper 2016. EPMA J. 2016;7:23. doi: 10.1186/s13167-016-0072-4. - DOI - PMC - PubMed
    1. Golubnitschaja O, Costigliola V, EPMA. General report & recommendations in predictive, preventive and personalised medicine 2012: white paper of the European Association for Predictive, Preventive and Personalised Medicine. EPMA J. 2012;3. 10.1186/1878-5085-3-14. - PMC - PubMed
    1. Einhorn L, Krapfenbauer K. HTRF: a technology tailored for biomarker determination—novel analytical detection system suitable for detection of specific autoimmune antibodies as biomarkers in nanogram level in different body fluids. EPMA J. 2015;6:23. doi: 10.1186/s13167-015-0046-y. - DOI - PMC - PubMed
    1. Lakshmi K, Nelakurthi H, Kumar A, Rudraraju A. Oral fluid-based biosensors: a novel method for rapid and noninvasive diagnosis. Indian J Dent Sci. 2017;9:60. doi: 10.4103/IJDS.IJDS_6_17. - DOI
    1. Pretty IA, Ellwood RP. The caries continuum: opportunities to detect, treat and monitor the re-mineralization of early caries lesions. J Dent. 2013;41(Supplement 2):S12–S21. doi: 10.1016/j.jdent.2010.04.003. - DOI - PubMed

LinkOut - more resources