doi: 10.1007/7651_2018_165.
Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells
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- PMID: 29896659
- DOI: 10.1007/7651_2018_165
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Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells
Methods Mol Biol.
2019.
Abstract
An overall three-dimensional picture of the distribution of epidermal cells at a given time point is of importance to better characterize epidermal progenitors. We introduce a whole-mount immunofluorescent staining coupled to a multicolor lineage tracing model for analyzing the spatiotemporal organization of epidermal stem cells. Laser scanning confocal microscopy with multiple labelling allows for robust imaging of dorsal skin with excellent resolution.
Keywords: Confocal microscopy; Mouse dorsal skin; Multicolor lineage tracing model; Whole-mount immunofluorescent staining.
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