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. 2018 Jun 1;29(6):1486-1488.
doi: 10.1093/annonc/mdy125.

Functional immune characterization of HIV-associated non-small-cell lung cancer

Affiliations

Functional immune characterization of HIV-associated non-small-cell lung cancer

D J Pinato et al. Ann Oncol. .
No abstract available

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Figures

Figure 1.
Figure 1.
(A–D) Representative sections of HIV-associated NSCLC co-immunostained for CD8 (red chromogen), CD4 (brown chromogen), PD-1 (blue chromogen) and CD68 (green chromogen) illustrating CD4/CD8 enrichment in PD-L1-positive HIV-associated NSCLC (A–C) compared with PD-L1-negative counterparts (B–D). Histograms of multiplex immunohistochemistry data illustrating the relationship between the CD4- and CD8-positive immune infiltrate expressed as number of immunopositive cells per high power field (HPF) according to tumour cell PD-L1 (E) and PD-L2 expression status (F) in HIV-associated NSCLC (n = 21). Peripheral blood immunophenotyping illustrates the relationship between peripheral CD4, CD8, CD19 and CD56-positive cell counts categorized according to tumoural PD-L1 (G) and PD-L2 expression status (H) in HIV-associated NSCLC (n = 24). Medians and interquartile ranges are reported. (I) Volcano plot of differentially regulated genes identified by Nanostring analysis. The Benjamini–Hockberg P-values are correlated to fold-changes in transcripts identified in HIV-positive NSCLC (n = 5) versus HIV-negative controls (n = 3). Transcripts achieving statistical significance (FDR q-value of 5%) are highlighted by the presence of the corresponding gene name. (J) Heat map profile of the 12 transcripts that are differentially regulated in HIV-associated NSCLC compared with controls. Expression levels are shown as log transformed values of normalized counts (ln2).

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