Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Feb;4(2):555-60.
doi: 10.1002/j.1460-2075.1985.tb03664.x.

dnaA protein-regulated transcription: effects on the in vitro replication of Escherichia coli minichromosomes

H Lother, R Kölling, C Kücherer, M Schauzu

dnaA protein-regulated transcription: effects on the in vitro replication of Escherichia coli minichromosomes

H Lother et al. EMBO J. 1985 Feb.

Abstract

Both initiation of replication and initiation of transcription are influenced by dnaA protein, when minichromosomes are assayed in vitro for dnaA protein complementation. This dnaA protein effect is seen only if minichromosomes are used containing the 16-kd promoter, from which transcription is directed into the minimal origin. Determination of the 16-kd promoter activity both in vivo and in vitro showed that this strong promoter is specifically repressed by dnaA protein. The 16-kd promoter is thus an integral regulatory region of oriC.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mol Gen Genet. 1970;109(2):107-22 - PubMed
    1. Annu Rev Genet. 1973;7:267-87 - PubMed
    1. J Mol Biol. 1975 May 5;94(1):127-32 - PubMed
    1. J Mol Biol. 1976 Apr 15;102(3):477-86 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed

Publication types