Isolation and characterization of cell clones producing various amounts of bovine leukosis virus
- PMID: 2991025
Isolation and characterization of cell clones producing various amounts of bovine leukosis virus
Abstract
Several single-cell clones were isolated from lamb kidney cell line (FLK) persistently infected with bovine leukosis virus (BLV). The clones differed in the amount of produced virus, some clones were highly virus productive, others produced less virus. The restriction analysis of cell DNA from clones revealed the existence of several numbers of integrated BLV-DNA proviruses in various cell clones. In some cell clones the unintegrated proviral DNA was observed. The proviral BLV sequences were found to be integrated in reiterated DNA in some cell clones. There was no apparent relationship between the number of integrated BLV proviruses and the extent of virus production. The origin of the different numbers of integrated BLV proviruses in various cell clones is discussed.
Similar articles
-
Bovine leukemia virus: isolation and characterization of nonproducer cell clones.Neoplasma. 1987;34(6):641-52. Neoplasma. 1987. PMID: 2829041
-
The morphogenesis of BLV (bovine leukemia virus) cultivated on FLK (fetal lamb kidney): an ultrastructural study.Microbiologica. 1988 Jan;11(1):1-5. Microbiologica. 1988. PMID: 2832708
-
Transfection of BLV containing DNA into NIH 3T3 cells.Neoplasma. 1985;32(6):663-71. Neoplasma. 1985. PMID: 3003601
-
Experimental transmission of the bovine leukaemia virus genome by cell contact.Folia Biol (Praha). 1987;33(6):400-9. Folia Biol (Praha). 1987. PMID: 2449369
-
Bovine leukaemia virus and enzootic bovine leukosis.Onderstepoort J Vet Res. 1985 Sep;52(3):133-44. Onderstepoort J Vet Res. 1985. PMID: 3003647 Review.
Cited by
-
Establishment of a new bovine leukosis virus producing cell line.J Virol Methods. 2004 Nov;121(2):239-46. doi: 10.1016/j.jviromet.2004.06.017. J Virol Methods. 2004. PMID: 15381362 Free PMC article.
-
Isolation and characterization of a 2.3-kilobase-pair cDNA fragment encoding the binding domain of the bovine leukemia virus cell receptor.J Virol. 1993 Feb;67(2):1050-7. doi: 10.1128/JVI.67.2.1050-1057.1993. J Virol. 1993. PMID: 8380453 Free PMC article.
-
Long-term infection with retroviral structural gene vector provides protection against bovine leukemia virus disease in rabbits.Virology. 2004 Nov 24;329(2):434-9. doi: 10.1016/j.virol.2004.09.001. Virology. 2004. PMID: 15518821 Free PMC article.
-
Polyclonal bovine sera but not virus-neutralizing monoclonal antibodies block bovine leukemia virus (BLV) gp51 binding to recombinant BLV receptor BLVRcp1.J Virol. 1997 Apr;71(4):3263-7. doi: 10.1128/JVI.71.4.3263-3267.1997. J Virol. 1997. PMID: 9060692 Free PMC article.
-
Conformational changes in DNA upon ligand binding monitored by circular dichroism.Int J Mol Sci. 2012;13(3):3394-3413. doi: 10.3390/ijms13033394. Epub 2012 Mar 12. Int J Mol Sci. 2012. PMID: 22489158 Free PMC article. Review.