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. 1978 Sep;8(3):320-5.
doi: 10.1128/jcm.8.3.320-325.1978.

Primary isolation media for Legionnaires disease bacterium

Primary isolation media for Legionnaires disease bacterium

J C Feeley et al. J Clin Microbiol. 1978 Sep.

Abstract

Yolk sac suspensions infected with the Legionnaires disease bacterium (LDB) were plated onto 17 different bacteriological agar media. The LDB grew only on Mueller-Hinton agar supplemented with 1% Iso Vitale X and 1% hemoglobin (MH-IH). This medium was subsequently analyzed to determine the components required to support growth of the LDB. L-Cysteine hydrochloride can replace the Iso Vitale X reagent, and soluble ferric pyrophosphate can replace hemoglobin. A new medium, F-G agar, was formulated incorporating these chemicals. Different cultures conditions (oxygen tension, temperature, and pH) were also evaluated. The LDB grew optimally at 35 degrees C under 2.5% CO2 on the F-G agar adjusted to pH 6.9. When infected tissues were inoculated onto both F-G agar and MH-IH, the F-G agar produced colonies of the LDB more rapidly and in greater numbers than did MH-IH.

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References

    1. Adv Protein Chem. 1956;11:311-427 - PubMed
    1. N Engl J Med. 1977 Dec 1;297(22):1189-97 - PubMed
    1. N Engl J Med. 1977 Dec 1;297(22):1197-203 - PubMed
    1. N Engl J Med. 1977 Dec 1;297(22):1218-20 - PubMed
    1. J Clin Microbiol. 1977 Aug;6(2):140-3 - PubMed

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