Primary isolation media for Legionnaires disease bacterium
- PMID: 29911
- PMCID: PMC275239
- DOI: 10.1128/jcm.8.3.320-325.1978
Primary isolation media for Legionnaires disease bacterium
Abstract
Yolk sac suspensions infected with the Legionnaires disease bacterium (LDB) were plated onto 17 different bacteriological agar media. The LDB grew only on Mueller-Hinton agar supplemented with 1% Iso Vitale X and 1% hemoglobin (MH-IH). This medium was subsequently analyzed to determine the components required to support growth of the LDB. L-Cysteine hydrochloride can replace the Iso Vitale X reagent, and soluble ferric pyrophosphate can replace hemoglobin. A new medium, F-G agar, was formulated incorporating these chemicals. Different cultures conditions (oxygen tension, temperature, and pH) were also evaluated. The LDB grew optimally at 35 degrees C under 2.5% CO2 on the F-G agar adjusted to pH 6.9. When infected tissues were inoculated onto both F-G agar and MH-IH, the F-G agar produced colonies of the LDB more rapidly and in greater numbers than did MH-IH.
Similar articles
-
Virulent to avirulent conversion of Legionnaires' disease bacterium (Legionella pneumophila)--its effect on isolation techniques.J Infect Dis. 1979 Jun;139(6):707-11. doi: 10.1093/infdis/139.6.707. J Infect Dis. 1979. PMID: 448196
-
Legionnaires' disease bacterium isolated in 1947.Ann Intern Med. 1979 Apr;90(4):659-61. doi: 10.7326/0003-4819-90-4-659. Ann Intern Med. 1979. PMID: 373548
-
Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila.J Clin Microbiol. 1979 Oct;10(4):437-41. doi: 10.1128/jcm.10.4.437-441.1979. J Clin Microbiol. 1979. PMID: 393713 Free PMC article.
-
[Bacteriology of Legionnaires' disease].Recenti Prog Med. 1981 May;70(5):557-8. Recenti Prog Med. 1981. PMID: 7031802 Review. Italian. No abstract available.
-
[Legionnaires' disease, a previously unknown infectious disease of bacterial nature].Zh Mikrobiol Epidemiol Immunobiol. 1979 Feb;(2):8-14. Zh Mikrobiol Epidemiol Immunobiol. 1979. PMID: 369246 Review. Russian. No abstract available.
Cited by
-
Identification and functional characterization of K(+) transporters encoded by Legionella pneumophila kup genes.Cell Microbiol. 2013 Dec;15(12):2006-19. doi: 10.1111/cmi.12168. Epub 2013 Aug 2. Cell Microbiol. 2013. PMID: 23848378 Free PMC article.
-
Legionella and Legionnaires' disease: 25 years of investigation.Clin Microbiol Rev. 2002 Jul;15(3):506-26. doi: 10.1128/CMR.15.3.506-526.2002. Clin Microbiol Rev. 2002. PMID: 12097254 Free PMC article. Review.
-
Amino acid requirements for Legionella pneumophila growth.J Clin Microbiol. 1981 May;13(5):865-9. doi: 10.1128/jcm.13.5.865-869.1981. J Clin Microbiol. 1981. PMID: 6113250 Free PMC article.
-
Immunological and biochemical relationships among flagella isolated from Legionella pneumophila serogroups 1, 2, and 3.Infect Immun. 1981 Aug;33(2):602-10. doi: 10.1128/iai.33.2.602-610.1981. Infect Immun. 1981. PMID: 6792082 Free PMC article.
-
Legionella pneumophila replication vacuoles mature into acidic, endocytic organelles.J Exp Med. 2000 Nov 6;192(9):1261-72. doi: 10.1084/jem.192.9.1261. J Exp Med. 2000. PMID: 11067875 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources