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. 2018 Jul;24(7):1285-1291.
doi: 10.3201/eid2407.171234.

Reemergence of Reston ebolavirus in Cynomolgus Monkeys, the Philippines, 2015

Catalino Demetria, Ina Smith, Titus Tan, Daniel Villarico, Edson Michael Simon, Rex Centeno, Mary Tachedjian, Satoshi Taniguchi, Masayuki Shimojima, Noel Lee J Miranda, Mary Elizabeth Miranda, Melissa Marie R Rondina, Rowena Capistrano, Amado Tandoc 3rd, Glenn Marsh, Debbie Eagles, Ramses Cruz, Shuetsu Fukushi

Reemergence of Reston ebolavirus in Cynomolgus Monkeys, the Philippines, 2015

Catalino Demetria et al. Emerg Infect Dis. 2018 Jul.

Abstract

In August 2015, a nonhuman primate facility south of Manila, the Philippines, noted unusual deaths of 6 cynomolgus monkeys (Macaca fascicularis), characterized by generalized rashes, inappetence, or sudden death. We identified Reston ebolavirus (RESTV) infection in monkeys by using serologic and molecular assays. We isolated viruses in tissues from infected monkeys and determined viral genome sequences. RESTV found in the 2015 outbreak is genetically closer to 1 of the 4 RESTVs that caused the 2008 outbreak among swine. Eight macaques, including 2 also infected with RESTV, tested positive for measles. Concurrently, the measles virus was circulating throughout the Philippines, indicating that the infection of the macaques may be a reverse zoonosis. Improved biosecurity measures will minimize the public health risk, as well as limit the introduction of disease and vectors.

Keywords: Reston Ebolavirus; coinfection; macaques; measles virus; nonhuman primates; the Philippines; viruses; zoonoses.

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Figures

Figure 1
Figure 1
Phylogenetic tree (neighbor-joining) of the full genomes of Ebola viruses and comparison to the Reston 2015 viruses DrpZ52BF (GenBank accession no. MF540570) and DrpZ210BG (GenBank accession no. MF540571) produced by using MEGA 6 software (https://www.megasoftware.net). Bold text indicates the genomes being sequenced. Numbers along branches indicate bootstrap values. Scale bar indicates nucleotide substitutions per site.
Figure 2
Figure 2
Immunofluorescence assay results of infected monkey serum A) characterized by granular staining pattern of HeLa cells and B) noninfected monkey serum. Original magnification ×400.
Figure 3
Figure 3
Phylogenetic tree (neighbor-joining) of the partial L gene (418 nt) of measles virus (GenBank accession no. MF496232) detected in macaques in 2015, produced by using MEGA 6 software (https://www.megasoftware.net). Numbers along branches indicate bootstrap values. Scale bar indicates nucleotide substitutions per site. Bold text indicates measles virus strain isolated in Philippines.
See this image and copyright information in PMC

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