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. 2018 Jun 15;16(6):211.
doi: 10.3390/md16060211.

Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite

Emna Ben Slimane  1   2 , Saloua Sadok  3
Affiliations

Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite

Emna Ben Slimane et al. Mar Drugs. .

Abstract

The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus). The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of α, β and γ chains. As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure. Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film. Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film. Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen. Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.

Keywords: cartilaginous fish by-products; chitosan; collagen; composite films; properties.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of (1): acid soluble collagen (ASC) and (2): pepsin soluble collagen (PSC) from hound-smooth skin M: high molecular weight marker (KDa).
Figure 2
Figure 2
Peptide mapping of acid soluble collagen ASC and pepsin soluble collagen PSC digested by Lysyl endopeptidase with different hydrolysis time 1: ASC, 2: ASC-5 min, 3: ASC-25 min, 4: PSC, 5: PSC-5 min, 6: PSC-25 min from hound smooth skin, M: high molecular weight marker (KDa).
Figure 3
Figure 3
Change in fractional viscosity with temperature of acid soluble collagen (ShS-ASC) and pepsin soluble collagen (ShS-PSC) from the skin of hound smooth.
Figure 4
Figure 4
Ultraviloet-Visible spectra of acid soluble (ShS-ASC) and pepsin soluble collagen (ShS-PSC) from the skin of hound smooth.
Figure 5
Figure 5
Fourier Transform Infrared spectra of ASC (acid soluble collagen) and PSC (pepsin soluble collagen) from the skin of hound smooth fish.
Figure 6
Figure 6
Pictures of (A) pure collagen film CO, (B) bi-composite Collagen-chitosan film and (C) pure chitosan film CH.
Figure 7
Figure 7
Optical transmission spectra of collagen films. CO: pure collagen film; CH: pure chitosan film; C50: Collagen-chitosan film 50%:50%, C75: Collagen-chitosan film 25%:75%.
Figure 8
Figure 8
Fourier transform infrared spectra of different collagen films, CO: pure collagen film; CH: pure chitosan film; C50: Collagen-chitosan film 50%:50%, C75: Collagen-chitosan film 25%:75%.
Figure 9
Figure 9
Collagen extraction process from smooth hound skin (ShS).
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