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. 1985 May;131(5):1130-40.
doi: 10.1099/00221287-131-5-1131.

Plasmid fusions mediated by one end of TnA

J Heritage, P M Bennett

Plasmid fusions mediated by one end of TnA

J Heritage et al. J Gen Microbiol. 1985 May.

Abstract

We have observed plasmid fusions in a recA background mediated by a single end of TnA. These occur when transposase is provided either in cis or in trans. Insertions of the plasmid carrying the TnA inverted repeat sequence occur at many sites in the target plasmid. The point of fusion on the plasmid carrying TnA sequences always appears to be located in the region which carries the TnA inverted repeat sequence. In contrast to the transposition of an intact TnA element, plasmid fusions mediated by one end of TnA are very rare events. The implications of our results for models of transposition are discussed.

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