Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Mar-Apr;23(3-4):163-9.
doi: 10.1016/0162-0134(85)85021-2.

Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization

C H Kim, M Radermacher, M Kessel, J Frank, T E King

Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization

C H Kim et al. J Inorg Biochem. 1985 Mar-Apr.

Abstract

Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0 degree projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 A. Our structural results are in agreement with the model of Henderson et al. [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.

PubMed Disclaimer

Publication types

LinkOut - more resources