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. 2018:1789:1-7.
doi: 10.1007/978-1-4939-7856-4_1.

GREEN FLUORESCENT SEED, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds

Tomoo Shimada  1 , Kentaro Fuji  1 , Takuji Ichino  1 , Ooi-Kock Teh  1 , Yasuko Koumoto  1 , Ikuko Hara-Nishimura  2   3
Affiliations

GREEN FLUORESCENT SEED, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds

Tomoo Shimada et al. Methods Mol Biol. 2018.

Abstract

Vacuolar trafficking plays a vital role in plant growth and development. In this chapter, we describe a powerful technique for the evaluation of vacuolar protein trafficking, which is designated as GREEN FLUORESCENT SEED. Based on vacuole-targeted green fluorescent protein in Arabidopsis seeds, this method enables the nondestructive isolation of mutant seeds defective in vacuolar trafficking and their visual characterization.

Keywords: Arabidopsis thaliana; GREEN FLUORESCENT SEED (GFS); Green fluorescent protein (GFP); Vacuolar sorting receptor 1 (VSR1); Vacuolar trafficking; Vacuole.

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