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. 2018 Nov;32(9):e22585.
doi: 10.1002/jcla.22585. Epub 2018 Jun 19.

Immunophenotypes and clinical features of lymphocytes in the labial gland of primary Sjogren's syndrome patients

Wenwen Sun  1 , Na Zhang  1 , Yujie Zhang  2 , Zonghong Shao  3 , Lu Gong  1 , Wei Wei  1
Affiliations

Immunophenotypes and clinical features of lymphocytes in the labial gland of primary Sjogren's syndrome patients

Wenwen Sun et al. J Clin Lab Anal. 2018 Nov.

Abstract

Objective: To investigate consistency of lymphocyte immunophenotype between labial gland and peripheral blood in patients with primary Sjogren's syndrome (pSS).

Methods: Seventy-one pSS patients and 35 patients with maxillofacial trauma were included in the study. Based on the ratio of CD20 to CD3 in labial gland from 71 pSS patients, they were divided into the high and (n = 48) and low CD20 expression group (n = 23). Lymphocyte immunophenotypes in labial glands, course of disease, erythrocyte sedimentation rate (ESR), C-reactive protein, immunoglobulin, and complement levels were analyzed.

Results: In the labial gland, the levels of IgG, IgA, IgM, and C3c were higher, but C1q was lower in the pSS group than in the control group (all P < .05). CD20 was detected in labial gland samples of all pSS patients, in which CD3 was positive in 66 (93.0%) patients, and negative in 5 (7.0%). The plasma levels of IgG, IgA, IgM, and CRP, and ESR were higher, but serum C4 level was lower in pSS patients than in the control group (all P < .01). Serum IgG level, ESR, and labial gland CD20 were higher in the high CD20 expression group than the low expression group (all P < .05).

Conclusion: Primary Sjogren's syndrome patients had a higher expression of CD20 positive infiltrating lymphocytes of the labial gland, accompanied with the changes of immunoglobulins, and complements in both the labial gland and peripheral blood.

Keywords: CD20; CD3; complement; immunoglobulin; labial gland; primary Sjogren's syndrome.

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Conflict of interest statement

All the authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
H&E staining of labial glands in PSS patients (×100): the salivary gland shows diffuse infiltration of ductal lymphoid tissue (including lymphocytes and plasma cells), irregular expansion, and peripheral acinar destruction with mild eosinophilic changes in the duct
Figure 2
Figure 2
Immunohistochemical staining of labial gland in pSS patients (×100): CD20 expression of lymphocyte tissue (A, showing more brown staining) was significantly higher than CD3 expression (B, showing brown staining less than A)
Figure 3
Figure 3
The interstitia of the labial gland in the control group showed negative immunohistochemical staining (×100): Scattered lymphocyte infiltration was found, but no positive staining for CD3 and Cd20 (brown color) was observed
Figure 4
Figure 4
Expresison of IgG in the labial gland of pSS paitents. Immunohistochemistry showed that the IgG level was apparently higher in the high CD20 expresison group (A) than the low CD20 expresison group (B). ×100
Figure 5
Figure 5
Expresison of C1q in the labial gland of pSS paitents. Immunohistochemistry showed that the C1q level was apparently higher in the high CD20 expresison group (A) than the low CD20 expresison group (B). ×100
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