[Efficiency Analysis of EX16+10Y Kit on Detection of the Uygur Population in Xinjiang Province]

Abstract

Objectives: To analyse the efficiency of EX16+10Y kit on the forensic detection of the Uygur in Xinjiang province.

Methods: The blood samples were extracted from 4 620 male individuals of Uygur in Xinjiang province, and amplified by EX16+10Y kit. The typing of amplification products was performed by 3130xl genetic analyzer.

Results: The genotyping graphs of 15 autosomal STR loci and 10 Y-chromosomal STR loci from 4 620 male individuals of Uygur in Xinjiang province were acquired completely. The genotype distribution of 15 autosomal STR loci was consistent with Hardy-Weinberg equilibrium. The heterozygosity, polymorphism information content and discrimination power of STR loci were 0.637-0.838, 0.580-0.860 and 0.811-0.978, respectively. There were 766 haplotypes in 10 Y -chromosomal STR loci.

Conclusions: The test results of EX16+10Y kit is accurate and trustworthy, which can simultaneously be used for the individual identification and the screening of paternal pedigree in practical work.

Keywords: Xinjiang; EX16+10Y kit; Uygur nationality; Y chromosome; forensic genetics; short tandem repeat.