doi: 10.1007/BF02798543.
Evidence for IFN-beta heterogeneity in a substrain of Namalwa cells
- PMID: 2992372
- DOI: 10.1007/BF02798543
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Evidence for IFN-beta heterogeneity in a substrain of Namalwa cells
Appl Biochem Biotechnol.
1985 Apr.
Abstract
A substrain of Namalwa cells, denoted substrain B, was grown in fermentors up to the 100-L scale, and was induced with Sendai virus to produce interferon (IFN). The titer of the crude IFN varied extensively between different batches; part of the variation was caused by a differential expression of IFN-alpha and IFN-beta. More than 80% of the IFN activity was IFN-beta by several criteria. A two-step purification procedure was developed and the resulting preparation had a specific activity of approximately 10(6) U/mg protein. The IFN-beta type was found to be heterogeneous, and could be separated into several components, which probably represented post-translational modifications of one molecule.
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