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. 2018 Aug 27;56(9):e00686-18.
doi: 10.1128/JCM.00686-18. Print 2018 Sep.

Xpert Ultra Can Unambiguously Identify Specific Rifampin Resistance-Conferring Mutations

Kamela C S Ng  1 , Armand van Deun  2   3 , Conor J Meehan  2 , Gabriela Torrea  2 , Michèle Driesen  2 , Siemon Gabriëls  2 , Leen Rigouts  2   4 , Emmanuel André #  5 , Bouke C de Jong #  2
Affiliations

Xpert Ultra Can Unambiguously Identify Specific Rifampin Resistance-Conferring Mutations

Kamela C S Ng et al. J Clin Microbiol. .
No abstract available

Keywords: Ultra probes; Xpert MTB/RIF Ultra; disputed mutations; melt peak temperature (Tm); melting temperature shift (ΔTm); rifampin-resistant tuberculosis; rpoB mutations.

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Figures

FIG 1
FIG 1
Overview of Xpert Ultra test results. The observed probe reactions for each RRDR mutation were laid over the claimed probe coverage (light gray). Shown in black are probe reactions concordant with manufacturer claims, in blue are probe reactions missed by one probe but captured by another probe, and in red is a probe reaction representing a “RIF Resistance Indeterminate” result from 3 out of 4 strains tested. Results in the hatched pattern were superimposed for greater visibility.
FIG 2
FIG 2
Melting temperature shifts (ΔTms) observed upon detection of a rifampin resistance (RR)-conferring rpoB mutation in the RR-determining region (RRDR) by Xpert Ultra. The y axis reflects the melting temperature difference (ΔTm) between mutant and wild-type probe-amplicon hybrids, while the x axis shows the mutations that we tested. The data points on the graph are ΔTm values grouped by their associated Ultra probes (differentiated by color), which correspond to a specific rpoB mutation. x axis labels in brown are disputed mutations.
See this image and copyright information in PMC

References

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