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. 1985;17(5):645-8.
doi: 10.1016/0020-711x(85)90298-8.

Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme

K Akai, K Yagi, H F Tiano, Y C Pan, M Shimizu, K Fong, R A Jungmann, S S Li

Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme

K Akai et al. Int J Biochem. 1985.

Abstract

A mouse lactate dehydrogenase-A cDNA was isolated and it was shown to contain the 393bp of the protein-coding sequence and 488bp of the 3' untranslated region. The amino acid sequence deduced from its open reading frame provided independent evidence for the sequence of residues 201-331 of mouse LDH-A subunit (muscle). This cDNA clone was used as a probe to isolate a mouse genomic clone containing a truncated, processed LDH-A pseudogene. This pseudogene showed 81.6% homology at 713 positions compared with the LDH-A cDNA sequence. The divergence of this pseudogene was estimated to have occurred 39 million years ago.

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