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. 2018 May 23:7:82.
doi: 10.4103/abr.abr_287_16. eCollection 2018.

A 53 KDa Glycan Antigen of Hydatid Cyst Wall May Involve in Evasion from Host Immune System

Raheleh Rafiei Sefiddashti  1 , Seyedeh Maryam Sharafi  2 , Soltan Ahmad Ebrahimi  3 , Lame Akhlaghi  1 , Ali Moosavi  4 , Abasali Eskandarian  5 , Hossein Yousofi Darani  5
Affiliations

A 53 KDa Glycan Antigen of Hydatid Cyst Wall May Involve in Evasion from Host Immune System

Raheleh Rafiei Sefiddashti et al. Adv Biomed Res. .

Abstract

Background: Recent studies have shown that similar host glycan antigens are expressed by helminths such as Echinococcus granulosus hydatid cysts to evade from host immune system. In this work to investigate these antigens further, immunological cross-reactivity between human sera and hydatid cyst wall antigens has been investigated.

Materials and methods: Hydatid cyst wall antigens were used in enzyme-linked immunosorbent assay and Western immunoblotting and probed with pooled sera of hydatidosis patients and healthy controls. Sodium metaperiodate treatment was used to investigate glycan antigens.

Results: A band with molecular weight about 53 KDa reacted with both hydatid patients' sera and also normal human sera. It has been shown that this band was a glycan antigen.

Conclusions: A 53 KDa glycan antigen of hydatid cyst wall that reacted with all human sera may have an important role for evasion from host immune system.

Keywords: Antigen; glycan; hydatid cyst; immune evasion.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Western immunoblotting of hydatid cyst wall antigen under reducing condition probed with pool sera from patients with hydatid cyst (2) or normal human sera (1)
Figure 2
Figure 2
Western immunoblotting of hydatid cyst wall before (2) and after (1) sodium metaperiodate treatment probed with sera of patients with hydatid cyst (H) or normal human sera (N)
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