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. 1985 Sep;163(3):913-7.
doi: 10.1128/jb.163.3.913-917.1985.

Mu-lac insertion-directed mutagenesis in a pectate lyase gene of Erwinia chrysanthemi

A Diolez, A Coleno

Mu-lac insertion-directed mutagenesis in a pectate lyase gene of Erwinia chrysanthemi

A Diolez et al. J Bacteriol. 1985 Sep.

Abstract

The pelC gene, which encodes one of the five major pectate lyase (PL) isoenzymes in Erwinia chrysanthemi 3937, designated PLc, was subcloned from a hybrid lambda phage into a pBR322 derivative and mutagenized with a mini-Mu-lacZ transposable element able to form fusions to the lacZ gene. One plasmid (pAD1) which had an inactivated pelC gene and a Lac+ phenotype was selected in Escherichia coli. This plasmid was introduced into Erwinia chrysanthemi, and the pelC::mini-Mu insertion was substituted for the chromosomal allele by homologous recombination. This strain lacks the PLc isoenzyme. This Erwinia chrysanthemi strain has a Lac+ phenotype that is inducible by polygalacturonate, as are the wild-type PL activities.

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References

    1. EMBO J. 1985 Mar;4(3):781-5 - PubMed
    1. Annu Rev Microbiol. 1972;26:389-426 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Jan;81(2):535-9 - PubMed
    1. J Bacteriol. 1977 Dec;132(3):862-9 - PubMed
    1. Anal Biochem. 1984 Jun;139(2):383-9 - PubMed

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