The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus
- PMID: 2993338
- PMCID: PMC7119584
- DOI: 10.1016/0166-0934(85)90116-8
The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus
Abstract
An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences.
Similar articles
-
DNA sequencing studies of genomic cDNA clones of avian infectious bronchitis virus.Adv Exp Med Biol. 1984;173:215-24. doi: 10.1007/978-1-4615-9373-7_21. Adv Exp Med Biol. 1984. PMID: 6331112 No abstract available.
-
Cloning and sequencing of 5' terminal sequences from avian infectious bronchitis virus genomic RNA.J Gen Virol. 1986 Feb;67 ( Pt 2):221-8. doi: 10.1099/0022-1317-67-2-221. J Gen Virol. 1986. PMID: 3003236
-
Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus.J Gen Virol. 1987 Jan;68 ( Pt 1):57-77. doi: 10.1099/0022-1317-68-1-57. J Gen Virol. 1987. PMID: 3027249
-
The complete nucleotide sequence of avian infectious bronchitis virus: analysis of the polymerase-coding region.Adv Exp Med Biol. 1987;218:15-29. doi: 10.1007/978-1-4684-1280-2_3. Adv Exp Med Biol. 1987. PMID: 2829522 Review. No abstract available.
-
Molecular biology of avian infectious bronchitis virus.Prog Vet Microbiol Immunol. 1989;5:65-82. Prog Vet Microbiol Immunol. 1989. PMID: 2560396 Review. No abstract available.
Cited by
-
Molecular characterization of three rabbit rotavirus strains.Arch Virol. 1988;98(3-4):253-65. doi: 10.1007/BF01322173. Arch Virol. 1988. PMID: 2831858
-
Nucleotide sequence of coronavirus TGEV genomic RNA: evidence for 3 mRNA species between the peplomer and matrix protein genes.Virus Res. 1989 Jun;13(2):87-100. doi: 10.1016/0168-1702(89)90008-7. Virus Res. 1989. PMID: 2549745 Free PMC article.
-
Biotinylated and radioactive cDNA probes in the detection by hybridization of bovine enteric coronavirus.Mol Cell Probes. 1988 Sep;2(3):209-23. doi: 10.1016/0890-8508(88)90005-9. Mol Cell Probes. 1988. PMID: 3221884 Free PMC article.
-
Enteric coronavirus TGEV: partial sequence of the genomic RNA, its organization and expression.Biochimie. 1987 Jun-Jul;69(6-7):591-600. doi: 10.1016/0300-9084(87)90178-7. Biochimie. 1987. PMID: 2825819 Free PMC article.
-
Synthesis of long cDNA from viral RNA template.Gene Anal Tech. 1988 May-Jun;5(3):57-61. doi: 10.1016/0735-0651(88)90017-9. Gene Anal Tech. 1988. PMID: 3192156 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources