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. 1985 Aug;22(2):286-91.
doi: 10.1128/jcm.22.2.286-291.1985.

Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3

R D Shaw, D L Stoner-Ma, M K Estes, H B Greenberg

Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3

R D Shaw et al. J Clin Microbiol. 1985 Aug.

Abstract

We prepared monoclonal antibodies against two serotypically distinct rotavirus strains: Wa, a serotype 1 virus of human origin, and rhesus rotavirus, a simian serotype 3 virus. Monoclonal antibodies which react specifically with VP7 of each serotype were identified by hemagglutination inhibition tests, plaque reduction neutralization studies, and solid-phase immunoassays which used wild-type and reassortant strains of rotavirus. An enzyme-linked immunoassay was designed which utilizes two of these antibodies to correctly identify serotype 1 and serotype 3 viruses.

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References

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