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. 2018 Jun 25;19(6):1503-1508.
doi: 10.22034/APJCP.2018.19.6.1503.

Diagnostic and Prognostic Value of Talin-1 and Midkine as Tumor Markers in Hepatocellular Carcinoma in Egyptian Patients

Aya H Mashaly  1 , Rokiah Anwar, Mohamed A Ebrahim, Laila A Eissa, Mamdouh M El Shishtawy
Affiliations

Diagnostic and Prognostic Value of Talin-1 and Midkine as Tumor Markers in Hepatocellular Carcinoma in Egyptian Patients

Aya H Mashaly et al. Asian Pac J Cancer Prev. .

Abstract

Background: Hepatocellular carcinoma (HCC) is a main cause of cancer death all over the world. Treatment and outcome of HCC based on its early diagnosis. This study was conducted to estimate the role of talin-1 and midkine in combination with total antioxidant capacity (TAC) as tumor markers in HCC patients. Methods: Serum levels of talin-1 and midkine were measured in 90 Egyptian subjects including 44 patients with HCC, 31patients with cirrhosis and 15 healthy controls using enzyme-linked immunosorbent assay (ELISA) technique. While a colorimetric method was used for measurement of TAC. Results: Serum talin-1 in HCC patients was significantly lower than that in patients with cirrhosis (P<0.001) and normal control (P<0.001). In addition, increased invasion and metastasis correlated with reduced talin-1 level. Serum midkine in HCC patients was significantly higher compared to cirrhotic patients (P<0.001) and normal control (P<0.001). Midkine at a cut off value of 1683 pg/ml showed a sensitivity of (81.82%) and specificity of (83.87%). While alpha-fetoprotein (AFP) at a cut off value of 200 ng/ml had a sensitivity of (52.27%), while specificity was (96.77%). Midkine was positive in 80.9% of HCC patients with negative AFP. Serum TAC was significantly decreased in HCC patients when compared with control group (P<0.001). Conclusion: Talin-1 may be implicated in the carcinogenesis and metastasis of HCC and can be used as a useful tumor marker for HCC. Midkine may be a potential diagnostic marker for HCC and may be used in addition to AFP to increase the sensitivity of HCC detection.

Keywords: Talin-1; Midkine; hepatocellular carcinoma; alpha; fetoprotein; total antioxidant capacity.

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Figures

Figure 1
Figure 1
Serum Talin-1 Concentration in Patients with Hepatocellular Carcinoma (HCC), Liver Cirrhosis and Control Subjects. $, Significance against control group; *, Significance against cirrhotic group.
Figure 2
Figure 2
Serum Midkine Level in Patients with Hepatocellular Carcinoma (HCC), Liver Cirrhosis and Control Subjects. $, Significance against control group; *, Significance against cirrhotic group.
Figure 3
Figure 3
Serum Total Antioxidant Capacity Level in Patients with Hepatocellular Carcinoma (HCC), Liver Cirrhosis and Control Subjects. $, Significance against control group.
Figure 4
Figure 4
ROC Curve for Talin-1, Midkine and AFP to Diagnose HCC Patients.
Figure 5
Figure 5
ROC Curve for Talin-1, Midkine and Midkine/talin-1 Ratio for Diagnosis of Hepatocellular Carcinoma (HCC).
See this image and copyright information in PMC

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References

    1. Ashworth RE, Wu J. Mammalian target of rapamycin inhibition in hepatocellular carcinoma. World J Hepatol. 2014;6:776–82. - PMC - PubMed
    1. Bruha R, Vitek L, Marecek Z, et al. Decreased serum antioxidant capacity in patients with Wilson disease is associated with neurological symptoms. J Inherit Metab Dis. 2012;35:541–8. - PubMed
    1. Cao G, Prior RL. Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clin Chem. 1998;44:1309–15. - PubMed
    1. Cavallaro U, Christofori G. Cell adhesion in tumor invasion and metastasis:loss of the glue is not enough. Biochim Biophys Acta. 2001;1552:39–45. - PubMed
    1. Dai LC. Midkine translocated to nucleoli and involved in carcinogenesis. World J Gastroenterol. 2009;15:412–6. - PMC - PubMed

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