Rec-dependent and Rec-independent recombination of plasmid-borne duplications in Escherichia coli K12
- PMID: 2993800
- DOI: 10.1007/BF00330768
Rec-dependent and Rec-independent recombination of plasmid-borne duplications in Escherichia coli K12
Abstract
Plasmidic recombination in E. coli K12 has been previously demonstrated to be dependent on the host rec genotype. The construction of plasmids that carry a duplication within an antibiotic-resistance gene is described. Recombination between the direct repeats recreates an active antibiotic-resistance gene, allowing quantitative analysis of recombination frequencies in a closely related set of E. coli K12 strains carrying various rec mutations. Using this system, intraplasmidic recombination of a duplication within the pBR322 tetracycline-resistance gene is shown to be rec-dependent while recombination of a similar duplication within the kanamycin-resistance gene of Tn903 is shown to be independent of recA, recB, recC, recE, recF and sbcB.
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