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Comparative Study
. 1985 Aug 26;13(16):5869-82.
doi: 10.1093/nar/13.16.5869.

Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor

Y Furutani, M Notake, M Yamayoshi, J Yamagishi, H Nomura, M Ohue, R Furuta, T Fukui, M Yamada, S Nakamura
Free PMC article
Comparative Study

Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor

Y Furutani et al. Nucleic Acids Res. .
Free PMC article

Abstract

DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino acid residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7907-11 - PubMed
    1. J Exp Med. 1972 Jul 1;136(1):128-42 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 - PubMed

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